Characterization of BshA, bacillithiol glycosyltransferase from Staphylococcus aureus and Bacillus subtilis.

FEBS Letters
Heather UptonMamta Rawat

Abstract

The first step during bacillithiol (BSH) biosynthesis involves the formation of N-acetylglucosaminylmalate from UDP-N-acetylglucosamine and l-malate and is catalyzed by a GT4 class glycosyltransferase enzyme (BshA). Recombinant Staphylococcus aureus and Bacillus subtilis BshA were highly specific and active with l-malate but the former showed low activity with d-glyceric acid and the latter with d-malate. We show that BshA is inhibited by BSH and similarly that MshA (first enzyme of mycothiol biosynthesis) is inhibited by the final product MSH.

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Citations

Oct 4, 2012·Organic Letters·Alexander P LamersRichard N Armstrong
Jul 18, 2015·Expert Review of Anti-infective Therapy·Varahenage R PereraKit Pogliano
Jul 3, 2015·Molecular Microbiology·Zuelay Rosario-CruzJeffrey M Boyd
Feb 23, 2013·Microbiology·Eugeni BeldaAntoine Danchin
Jul 31, 2013·Microbiology·Ahmed GaballaJohn D Helmann
Apr 27, 2013·Biochemical and Biophysical Research Communications·Arishma RajkarnikarMamta Rawat
Oct 19, 2012·Biochimica Et Biophysica Acta·Robert C Fahey
Mar 18, 2017·Antioxidants & Redox Signaling·Pete ChandrangsuJohn D Helmann
Oct 30, 2013·Infection and Immunity·Ana C PosadaAmbrose Cheung
Jun 23, 2016·Chembiochem : a European Journal of Chemical Biology·Sunil V SharmaChris J Hamilton
Jun 30, 2016·Pathogens and Disease·William N Beavers, Eric P Skaar
May 10, 2020·Antioxidants·Mamta Rawat, Julie A Maupin-Furlow

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