Characterization of C1q found in a patient with hypocomplementemic vasculitis-urticaria syndrome

Microbiology and Immunology
T SasakiK Nagaki

Abstract

C1q, a subcomponent of the first complement component, of a 60-year-old female patient with hypocomplementemic vasculitis-urticaria syndrome (HVUS) was characterized. The C1q-precipitin activity (C1q-p) could not be detected by a routine method with 0.6% agarose in 10 mM Na-phosphate buffer containing 10 mM EDTA (pH 7.2). Hemolytic activity of her serum complement (CH50) and levels of C1 and C4 were significantly reduced at the exacerbation stage, but levels of other complement components were almost within the normal range throughout her clinical course. The specific activity of C1q at her exacerbation stage was significantly low, and its elution position on Sephacryl S-300 column was spread toward the low molecular weight in comparison with that of normal plasma. Molecular weights of the delayed fraction of C1q were estimated to be approximately 300,000 on the Sephacryl and 440,000 by the polyacrylamide gel electrophoresis (PAGE) containing sodium dodecyl sulfate (SDS) followed by immunoblotting, respectively. On reduction of her plasma, two bands with molecular weights equivalent to those of B and C chains of the normal C1q in an approximate molar ratio of 2:1 were immunostained. Plasma at her exacerbation stage showed only ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1979·Advances in Protein Chemistry·R R Porter, K B Reid
Dec 1, 1975·Journal of Immunological Methods·K Yonemasu
Sep 1, 1976·The Biochemical Journal·I GigliR B Sim
Apr 1, 1971·The Journal of Clinical Investigation·M M De Bracco, R M Stroud
May 1, 1972·Immunochemistry·K Yonemasu, R M Stroud
Aug 1, 1974·Journal of Immunological Methods·K NagakiS Inai
Sep 1, 1965·Immunochemistry·G ManciniJ F Heremans
Jul 3, 1980·The New England Journal of Medicine·R A ThompsonA H Cameron
Jan 1, 1982·Cold Spring Harbor Symposia on Quantitative Biology·E UngewickellD Branton
Feb 1, 1982·Clinical Immunology and Immunopathology·J O MintaM Greenberg
Sep 1, 1981·Annals of Internal Medicine·H NishinoM Mushimoto
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS

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Citations

Nov 20, 2009·Current Rheumatology Reports·Luis J JaraMiguel A Saavedra

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