Characterization of Caco-2 and HT29-MTX cocultures in an in vitro digestion/cell culture model used to predict iron bioavailability

The Journal of Nutritional Biochemistry
Gretchen J MahlerRaymond P Glahn

Abstract

Cocultures of two human cell lines, Caco-2 and HT29-MTX mucus-producing cells, have been incorporated into an in vitro digestion/cell culture model used to predict iron bioavailability. A range of different foods were subjected to in vitro digestion, and iron bioavailability from digests was assessed with Caco-2, Caco-2 overlaid with porcine mucin, HT29-MTX or cocultures of Caco-2 and HT29-MTX at varying ratios. It was found that increasing the ratio of HT29-MTX cells decreased the amount of ferritin formed and resulted in an overall decline in the ability of the model to detect differences in iron bioavailability. At the physiologically relevant ratios of 90% Caco-2/10% HT29-MTX and 75% Caco-2/25% HT29-MTX, however, a mucus layer completely covered the cell monolayer and the in vitro digestion model was nearly as responsive to changes in sample iron bioavailability as pure Caco-2 cultures. The in vitro digestion/Caco-2 cell culture model correlates well with human iron bioavailability studies, but, as mucus appears to play a role in iron absorption, the addition of a physiologically realistic mucus layer and goblet-type cells to this model may give more accurate iron bioavailability predictions.

Citations

Jan 5, 2012·Annals of Biomedical Engineering·Jong Hwan Sung, Michael L Shuler
Apr 25, 2012·Annals of Biomedical Engineering·Michael L Shuler
Mar 26, 2011·Genes & Nutrition·Michael D Garrick
Oct 15, 2013·International Journal of Pharmaceutics·Francisca Araújo, Bruno Sarmento
Mar 3, 2012·Journal of Pediatric Gastroenterology and Nutrition·Maciej ChichlowskiDavid A Mills
Jan 15, 2014·Advanced Drug Delivery Reviews·Mandy B EschMichael L Shuler
Feb 19, 2013·Advanced Drug Delivery Reviews·Jennifer M Gamboa, Kam W Leong
Apr 8, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Arnaud BéduneauAlf Lamprecht
Apr 26, 2011·Annual Review of Biomedical Engineering·M B EschM L Shuler
Jul 22, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Joost WesterhoutHeleen M Wortelboer
Feb 19, 2016·Particle and Fibre Toxicology·Anastasia GeorgantzopoulouArno C Gutleb
Aug 25, 2015·International Journal of Food Sciences and Nutrition·Fengguang PanJingbo Liu
Jun 15, 2010·Expert Opinion on Drug Metabolism & Toxicology·Jong Hwan SungMichael L Shuler
Nov 20, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Filipa AntunesBruno Sarmento
Feb 24, 2016·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Rishad R JivaniBhagyesh P Jhala
Dec 15, 2011·Advanced Drug Delivery Reviews·Shilpa SantAli Khademhosseini
Feb 18, 2010·Journal of Pharmacological and Toxicological Methods·Xiu-Min ChenDavid D Kitts
Nov 7, 2015·International Journal of Pharmaceutics·Peiwen ZhangYuan Huang
May 7, 2009·Biotechnology and Bioengineering·Gretchen J MahlerMichael L Shuler
Mar 25, 2016·Therapeutic Delivery·Jeffrey P PearsonMatthew D Wilcox
Mar 29, 2014·Particle and Fibre Toxicology·Emilie BrunMarie Carrière
Jan 21, 2015·International Journal of Pharmaceutics·Delphine AntoineArnaud Béduneau
May 6, 2014·Toxicology Letters·G JanerS Vázquez-Campos
May 3, 2014·Drug Discovery Today·Udita AgrawalSuresh P Vyas
Feb 5, 2014·Drug Discovery Today·Anne-Claire Groo, Frederic Lagarce
Apr 22, 2014·The Journal of Nutritional Biochemistry·Nathalie M ScheersAnn-Sofie Sandberg
May 23, 2014·Trends in Biotechnology·Lucas T VuPadmavathy Rajagopalan
Oct 5, 2014·Applied Microbiology and Biotechnology·Adrien NivoliezStéphanie Bornes
Jun 14, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Abhijit A DateLaura M Ensign
Jun 28, 2016·Archives of Toxicology·Eleonore Fröhlich, Eva Roblegg
Jul 22, 2016·Journal of Applied Toxicology : JAT·P ZellerE Leclerc
Aug 16, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Oljora RezhdoRebecca Carrier
May 27, 2017·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hans BouwmeesterMark A Jepson

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