Characterization of Campylobacter jejuni RacRS reveals roles in the heat shock response, motility, and maintenance of cell length homogeneity.

Journal of Bacteriology
Dmitry ApelErin C Gaynor

Abstract

Campylobacter jejuni commensally colonizes the cecum of birds. The RacR (reduced ability to colonize) response regulator was previously shown to be important in avian colonization. To explore the means by which RacR and its cognate sensor kinase RacS may modulate C. jejuni physiology and colonization, ΔracR and ΔracS mutations were constructed in the invasive, virulent strain 81-176, and extensive phenotypic analyses were undertaken. Both the ΔracR and ΔracS mutants exhibited a ~100-fold defect in chick colonization despite no (ΔracS) or minimal (ΔracR) growth defects at 42 °C, the avian body temperature. Each mutant was defective for colony formation at 44°C and in the presence of 0.8% NaCl, both of which are stresses associated with the heat shock response. Promoter-reporter and real-time quantitative PCR (RT-qPCR) analyses revealed that RacR activates racRS and represses dnaJ. Although disregulation of several other heat shock genes was not observed at 38°C, the ΔracR and ΔracS mutants exhibited diminished upregulation of these genes upon a rapid temperature upshift. Furthermore, the ΔracR and ΔracS mutants displayed increased length heterogeneity during exponential growth, with a high proportion of filamented bacteria. Fila...Continue Reading

References

Oct 1, 1992·Journal of Bacteriology·W ShiC A Gross
Nov 1, 1992·Journal of Medical Microbiology·P H EverestP H Williams
Dec 1, 1989·Microbiological Reviews·J B StockA M Stock
Aug 1, 1988·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·W StelzerH J Dobberkau
Oct 1, 1988·Applied and Environmental Microbiology·J T BeeryM P Doyle
Jun 1, 1988·Infection and Immunity·M B HugdahlM P Doyle
Jul 21, 1972·Science·K Schmidt-Nielsen
Jul 1, 1966·The American Journal of Digestive Diseases·J S Fordtran, T W Locklear
Nov 1, 1995·Infection and Immunity·C M SzymanskiG D Armstrong
Mar 1, 1997·Molecular Microbiology·R YaoP Guerry
Jun 1, 1997·Molecular Microbiology·K M Ottemann, J F Miller
Dec 13, 1997·The Journal of Infectious Diseases·M J Blaser
Feb 11, 1998·Journal of Bacteriology·M M WöstenB A van der Zeijst
Mar 12, 1998·Infectious Disease Clinics of North America·B M Allos
Jul 23, 1998·Infection and Immunity·M E KonkelR Ziprin
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·W C SuhC A Gross
Jan 8, 1999·Journal of Medical Microbiology·M ShigematsuK Amako
Mar 19, 1999·Emerging Infectious Diseases·S F AltekruseD L Swerdlow
Dec 14, 1999·Molecular Microbiology·A A Bianchi, F Baneyx
Apr 18, 2000·Infection and Immunity·A P Teixeira-GomesM S Zygmunt
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Apr 3, 2001·Journal of Clinical Microbiology·A C CarvalhoL K Pickering
Apr 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·B M Allos
Apr 9, 2001·Journal of Bacteriology·A JagannathanC W Penn
Jun 16, 2001·Advances in Microbial Physiology·P A Lund
Dec 12, 2002·Cellular and Molecular Life Sciences : CMLS·D A DouganB Bukau
Feb 13, 2004·The Journal of Biological Chemistry·Marc M S M WöstenJos P M van Putten

❮ Previous
Next ❯

Citations

Apr 9, 2014·Environmental Microbiology·Anne-Xander van der StelMarc M S M Wösten
Jun 28, 2016·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Tsuyoshi YamamotoHirofumi Nakano
Apr 9, 2020·Biomolecules·Shuichi Nakamura
Jan 11, 2020·Immune Network·Amber Hameed

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.