Characterization of Canine Adipose-Derived Mesenchymal Stromal/Stem Cells in Serum-Free Medium

Tissue Engineering. Part C, Methods
Zhuoming LiuLax R Devireddy

Abstract

In this article, we report on the development of a defined serum-free medium capable of supporting the culture expansion of mesenchymal stromal/stem cells (MSCs) from canine adipose tissue (canine Ad-MSCs). The potential benefits of serum-free media can only be utilized if cells cultured in serum-free media maintain the same functional characteristics as cells cultured in serum-containing media. Therefore, we analyze the characteristics of canine Ad-MSCs cultured in this serum-free medium or in serum-containing medium through evaluation of growth kinetics, clonogenic capacity, senescence, and differentiation capacity. Both, serum-containing medium and our serum-free medium, supported efficient growth and colony formation of canine Ad-MSCs. In addition, canine Ad-MSCs cultured in both media demonstrated similar viability after freeze/thaw, similar cell surface marker expression, and were capable of trilineage differentiation. While canine Ad-MSCs cultured in both media were generally similar, under the conditions of our study, canine Ad-MSCs cultured in serum-free medium demonstrated a shorter lag phase and higher colony-forming capacity, accelerated population doubling, maintained multipotentiality at higher passage numbers, an...Continue Reading

References

Mar 29, 2008·Methods in Molecular Biology·Radhika Pochampally
Apr 9, 2010·Molecular Biology of the Cell·Patricia A Zuk
Mar 5, 2011·Stem Cell Research & Therapy·Lisa A Fortier, Alexander J Travis
May 14, 2011·Transfusion Medicine and Hemotherapy : Offizielles Organ Der Deutschen Gesellschaft Fur̈ Transfusionsmedizin Und Immunham̈atologie·Charles J Hunt
Jul 12, 2011·Journal of Tissue Engineering and Regenerative Medicine·Sunghoon JungLeo A Behie
Jun 2, 2012·The Journal of Biological Chemistry·Hong QianMikael Sigvardsson
Aug 21, 2012·PloS One·Natalie FeketeHubert Schrezenmeier
Apr 13, 2013·Nature·David Cyranoski
Oct 15, 2013·Cell Proliferation·S GottipamulaR N Seetharam
Jul 23, 2016·International Journal of Molecular Sciences·Valentina TurinettoClaudia Giachino
Feb 2, 2017·Tissue Engineering. Part B, Reviews·Lax R DevireddyRudell Screven

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
light microscopy
FACS
fluorescence-activated cell sorting

Software Mentioned

GraphPad Prism
IncuCyte Zoom

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.