Characterization of carboxypeptidase A6, an extracellular matrix peptidase

The Journal of Biological Chemistry
Peter J LyonsL D Fricker

Abstract

Carboxypeptidase A6 (CPA6) is a member of the M14 metallocarboxypeptidase family that is highly expressed in the adult mouse olfactory bulb and broadly expressed in embryonic brain and other tissues. A disruption in the human CPA6 gene is linked to Duane syndrome, a defect in the abducens nerve/lateral rectus muscle connection. In this study the cellular distribution, processing, and substrate specificity of human CPA6 were investigated. The 50-kDa pro-CPA6 is routed through the constitutive secretory pathway, processed by furin or a furin-like enzyme into the 37-kDa active form, and secreted into the extracellular matrix. CPA6 cleaves the C-terminal residue from a range of substrates, including small synthetic substrates, larger peptides, and proteins. CPA6 has a preference for large hydrophobic C-terminal amino acids as well as histidine. Peptides with a penultimate glycine or proline are very poorly cleaved. Several neuropeptides were found to be processed by CPA6, including Met- and Leu-enkephalin, angiotensin I, and neurotensin. Whereas CPA6 converts enkephalin and neurotensin into forms known to be inactive toward their receptors, CPA6 converts inactive angiotensin I into the biologically active angiotensin II. Taken toge...Continue Reading

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Citations

Sep 22, 2010·The Journal of Biological Chemistry·Peter J Lyons, Lloyd D Fricker
Oct 30, 2012·The Journal of Biological Chemistry·Matthew R SapioLloyd D Fricker
Jan 12, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Iryna BerezniukLloyd D Fricker
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Jun 12, 2020·Neuropharmacology·Kinga HartmanJerzy Silberring

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