Characterization of Caulobacter crescentus FtsZ protein using dynamic light scattering.

The Journal of Biological Chemistry
Sen HouPiotr Garstecki

Abstract

The self-assembly of the tubulin homologue FtsZ at the mid-cell is a critical step in bacterial cell division. We introduce dynamic light scattering (DLS) spectroscopy as a new method to study the polymerization kinetics of FtsZ in solution. Analysis of the DLS data indicates that the FtsZ polymers are remarkably monodisperse in length, independent of the concentrations of GTP, GDP, and FtsZ monomers. Measurements of the diffusion coefficient of the polymers demonstrate that their length is remarkably stable until the free GTP is consumed. We estimated the mean size of the FtsZ polymers within this interval of stable length to be between 9 and 18 monomers. The rates of FtsZ polymerization and depolymerization are likely influenced by the concentration of GDP, as the repeated addition of GTP to FtsZ increased the rate of polymerization and slowed down depolymerization. Increasing the FtsZ concentration did not change the size of FtsZ polymers; however, it increased the rate of the depolymerization reaction by depleting free GTP. Using transmission electron microscopy we observed that FtsZ forms linear polymers in solutions which rapidly convert to large bundles upon contact with surfaces at time scales as short as several second...Continue Reading

References

Jan 1, 1985·Annual Review of Biophysics and Biophysical Chemistry·O G Berg, P H von Hippel
Feb 1, 1967·Biopolymers·V BloomfieldK E Van Holde
Feb 19, 1998·Journal of Colloid and Interface Science·P J Wyatt
Jun 17, 1998·Nature Structural Biology·E NogalesJ Löwe
Jun 27, 2000·Journal of Bacteriology·E L WhiteD W Borhani
Nov 15, 2000·The Journal of Cell Biology·J KiesslingR Reski
Jun 13, 2001·Molecular Microbiology·E J Harry
Nov 26, 2002·The Journal of Biological Chemistry·Tushar K BeuriaDulal Panda
Apr 18, 2003·Journal of Bacteriology·Richard B Weart, Petra Anne Levin
Oct 7, 2003·Annual Review of Microbiology·Laura Romberg, Petra Anne Levin
Jul 13, 2004·Virology·Greg L CasiniDavid T Wu
Aug 4, 2004·Proceedings of the National Academy of Sciences of the United States of America·Danielle N MargalitDebabrata RayChaudhuri
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·José Manuel GonzálezGermán Rivas
Mar 29, 2005·The Journal of Biological Chemistry·Jesús MingoranceMarisela Vélez
Jul 12, 2005·Current Biology : CB·Nathan W Goehring, Jon Beckwith
Jul 24, 2007·The Journal of Biological Chemistry·Yaodong ChenHarold P Erickson
Mar 12, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Loic HamonDavid Pastré
Feb 9, 2010·The Journal of Biological Chemistry·David PoppRobert C Robinson
Mar 10, 2010·The Journal of Biological Chemistry·José M AndreuAntonio J Martín-Galiano
Nov 3, 2010·The Journal of Organic Chemistry·Nohemy A SortoJared T Shaw
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Harold P EricksonMasaki Osawa
Apr 23, 2011·Biochemistry·Yaodong Chen, Harold P Erickson

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May 11, 2016·International Journal of Biological Macromolecules·Fan YangJianxi Xiao
Jul 5, 2013·The Journal of Biological Chemistry·Sara L Milam, Harold P Erickson
Nov 2, 2017·The Journal of Biological Chemistry·Kousik Sundararajan, Erin D Goley
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