Characterization of cell-cycle progression and growth of WB-F344 normal rat liver epithelial cells following gamma-ray exposure

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Bogdan I GerashchenkoRoger W Howell

Abstract

Apparently normal rat liver epithelial cells (WB-F344) have been widely used in studies pertaining to carcinogenesis. Ionizing radiation, a well known carcinogen, is known to perturb cell-cycle progression in a dose-dependent manner, thereby causing delay in cell proliferation. However, for WB-F344 cells, there is a paucity of such data, which are of substantial importance in understanding their radiation response. Here, the distribution of phases in the cell-cycle and the proliferation ability of WB-F344 cells are characterized at various time points after the cells have been irradiated with different doses of gamma-rays. After WB-F344 cells reached 100% confluence, they were trypsinized and suspended at 3.5 x 10(5) cells/ml in culture medium. Cells were irradiated in suspension with (137)Cs gamma-rays at doses from 1-10 Gy. After irradiation, 1 x 10(5) cells were plated into 60 x 15-mm culture dishes and incubated at 37 degrees C, with 2% CO(2) and 98% air. At 12, 24, 36, 48, and 60 h postirradiation, cells were harvested, counted, and subjected to flow cytometric cell-cycle analysis. Growth curves of WB-F344 cells irradiated with gamma-rays started to separate at 36 h postirradiation. By 60 h postirradiation, the growth curv...Continue Reading

References

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Jun 24, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bogdan I Gerashchenko, Roger W Howell
Aug 1, 1961·Proceedings of the National Academy of Sciences of the United States of America·M YAMADA, T T PUCK
Nov 11, 2003·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bogdan I Gerashchenko, Roger W Howell

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Citations

May 26, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Bogdan I Gerashchenko, Roger W Howell
Dec 13, 2006·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Paolo SportolettiMauro Di Ianni
Nov 29, 2016·Cellular and Molecular Life Sciences : CMLS·Katia FettucciariGabrio Bassotti
May 15, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Veronica CeccarelliAlba Vecchini
Dec 17, 2019·Problemy Radiat︠s︡iĭnoï Medyt︠s︡yny Ta Radiobiolohiï·B I GerashchenkoA M Babsky
Oct 9, 2018·Biochimica Et Biophysica Acta. Molecular Cell Research·Katia FettucciariGabrio Bassotti

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