Abstract
Apparently normal rat liver epithelial cells (WB-F344) have been widely used in studies pertaining to carcinogenesis. Ionizing radiation, a well known carcinogen, is known to perturb cell-cycle progression in a dose-dependent manner, thereby causing delay in cell proliferation. However, for WB-F344 cells, there is a paucity of such data, which are of substantial importance in understanding their radiation response. Here, the distribution of phases in the cell-cycle and the proliferation ability of WB-F344 cells are characterized at various time points after the cells have been irradiated with different doses of gamma-rays. After WB-F344 cells reached 100% confluence, they were trypsinized and suspended at 3.5 x 10(5) cells/ml in culture medium. Cells were irradiated in suspension with (137)Cs gamma-rays at doses from 1-10 Gy. After irradiation, 1 x 10(5) cells were plated into 60 x 15-mm culture dishes and incubated at 37 degrees C, with 2% CO(2) and 98% air. At 12, 24, 36, 48, and 60 h postirradiation, cells were harvested, counted, and subjected to flow cytometric cell-cycle analysis. Growth curves of WB-F344 cells irradiated with gamma-rays started to separate at 36 h postirradiation. By 60 h postirradiation, the growth curv...Continue Reading
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