Characterization of Cell-Death-Inducing Members of the Pectate Lyase Gene Family in Phytophthora capsici and Their Contributions to Infection of Pepper

Molecular Plant-microbe Interactions : MPMI
Li FuXiu-Guo Zhang

Abstract

Pectate lyases (PL) play a critical role in pectin degradation. PL have been extensively studied in major bacterial and fungal pathogens of a wide range of plant species. However, the contribution of PL to infection by oomycete pathogens remains largely unknown. Here, we cloned 22 full-length pectate lyase (PcPL) genes from a highly aggressive strain of Phytophthora capsici SD33. Of these, PVX agroinfiltration revealed that 12 PcPL genes were found to be highly induced during infection of pepper by SD33 but the induction level was twofold less in a mildly aggressive strain, YN07. The four genes with the highest transcript levels as measured by by quantitative reverse-transcription polymerase chain reaction (PcPL1, PcPL15, PcPL16, and PcPL20) also produced a severe cell death response following transient expression in pepper leaves but the other eight PcPL genes did not. Overexpression of these four genes increased the virulence of SD33 on pepper slightly, and increased it more substantially during infection of tobacco. Overexpression of the genes in YN07 restored its aggressiveness to near that of SD33. Gene silencing experiments with the 12 PcPL genes produced diverse patterns of silencing of PcPL genes, from which it could be...Continue Reading

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Citations

Jun 22, 2019·Annual Review of Microbiology·Yan WangYuanchao Wang
May 1, 2019·Molecular Plant-microbe Interactions : MPMI·Zhiqin LiuShuilin He
Jun 3, 2020·Molecular Plant-microbe Interactions : MPMI·Silvia RodríguezEduardo A Espeso
Dec 18, 2020·Frontiers in Plant Science·Jing LiuDeliang Peng

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