Characterization of cell lines developed from the Colorado potato beetle, Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae)

In Vitro Cellular & Developmental Biology. Animal
G CharpentierS Belloncik

Abstract

In order to isolate new pathogens (viruses, microsporidia, etc.) or to evaluate the efficiency of some pathogens (serovarieties and mutants of Bacillus thuringiensis, fungi, etc.) in the control of Colorado potato beetle, an economically important pest, we established four cell lines from tissues of this insect. One was initiated from embryonated egg fragments in the M3 medium supplemented with 20% fetal bovine serum (FBS) and then transferred after several passages to the Ex-Cell 400 medium with 20% FBS. Another was initiated from larval hemocytes in Ex-Cell 400 with 5% FBS. Finally, two other cell lines were initiated from adult hemocytes: one in the Ex-Cell 400 with 20% FBS and 1% of lipid mixture and the other in the Ex-Cell 400 with 5% FBS only. These cell lines have been characterized by their morphology with light and electron microscopy, their karyotypes, cell growth, and isozyme analysis. Each cell line differed in morphologic, karyologic, growth, and isozyme patterns. The cell line initiated from embryonated eggs was growing slower than the three initiated from hemocytes. The cytotoxicity of solubilized crystal delta-endotoxins from different B. thuringiensis formulations (M-One, Trident, MYX-1806, Teknar-HPD, and Thu...Continue Reading

References

Feb 1, 1995·In Vitro Cellular & Developmental Biology. Animal·D E Lynn
Nov 1, 1995·Journal of Medical Entomology·G CharpentierJ M Quiot
Jan 1, 1996·Archives of Insect Biochemistry and Physiology·C A Sheppard, D E Lynn
Sep 4, 1999·In Vitro Cellular & Developmental Biology. Animal·J L GringortenL Masson
Mar 1, 1999·Cytotechnology·X LéryS Belloncik

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Citations

Oct 24, 2008·In Vitro Cellular & Developmental Biology. Animal·Keita HoshinoKikuo Iwabuchi
Nov 15, 2011·Biotechnology Advances·Bing-Lan Liu, Yew-Min Tzeng
Oct 4, 2014·In Vitro Cellular & Developmental Biology. Animal·Xin ZhangCheng-Ye Wang
Jul 4, 2012·In Vitro Cellular & Developmental Biology. Animal·Cynthia L GoodmanYoonseong Park
Feb 24, 2017·In Vitro Cellular & Developmental Biology. Animal·Norichika Ogata, Kikuo Iwabuchi
Nov 5, 2020·In Vitro Cellular & Developmental Biology. Animal·Bhumi ThakkarPragna Parikh

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Methods Mentioned

BETA
electron microscopy
protein assay

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