PMID: 7543080Jul 28, 1995Paper

Characterization of cell lines established from human hepatocellular carcinoma

International Journal of Cancer. Journal International Du Cancer
J H ParkK U Lee

Abstract

We characterized 8 human hepatocellular-carcinoma cell lines established from the primary tumors of Korean patients. All lines showed substrate adherence and one line from anaplastic tumor also grew as floating aggregates. Most cultured cells maintained many morphological characteristics of the original tumors from which they were derived. Doubling times varied from 34 to 72 hr. All lines showed relatively high viability and were not contaminated with Mycoplasma or bacteria. All lines showed aneuploidy and were proven to be unique by DNA fingerprinting analysis. Hepatitis-B-virus (HBV) DNA was integrated in the genomes of all lines. Two of the cell lines (SNU-354, SNU-368) showed expression of HBV and HBVx (HBx) transcripts. SNU-354 strongly expressed albumin, and SNU-368 expressed transferrin and insulin-like growth factor II. No lines produced alpha-fetoprotein at the RNA and protein level. These cell lines represent useful tools for in vitro studies related to hepatocellular carcinoma.

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Citations

Jun 11, 1999·International Journal of Cancer. Journal International Du Cancer·J H OhJ G Park
Jul 15, 1999·International Journal of Cancer. Journal International Du Cancer·E C ShinS J Kim
Jun 19, 2001·International Journal of Cancer. Journal International Du Cancer·E C ShinJ H Park
May 23, 1998·In Vitro Cellular & Developmental Biology. Animal·W K LeeJ G Park
Dec 5, 2002·Archives of Biochemistry and Biophysics·Tae-Wook ChungCheorl-Ho Kim
Feb 20, 1999·Cancer Letters·E C ShinS J Kim
Mar 1, 2012·World Journal of Gastroenterology : WJG·Geum-Youn GwakByung Chul Yoo
Feb 1, 2005·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Ja-Lok Ku, Jae-Gahb Park
Mar 1, 2007·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Mi Hee KwackYoung Kwan Sung
Aug 1, 2007·Biomarkers in Medicine·Jeng-Eing JengJung-Fa Tsai
Jul 21, 2014·Cancer Chemotherapy and Pharmacology·Lynn Y L HuangJohnson Y N Lau
Apr 29, 2009·Journal of Cancer Research and Clinical Oncology·Long WuYan Li
Jul 29, 2008·Cancer Letters·Su Cheol ParkHyo-Suk Lee
Dec 11, 2007·Cancer Genetics and Cytogenetics·Yoshikazu InagakiTakeshi Okanoue
Jul 15, 2015·Molecular Oncology·Yuting KuangNouri Neamati
Jul 16, 2008·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tomoaki NakajimaTakeshi Okanoue
Apr 20, 2012·International Journal of Cancer. Journal International Du Cancer·Zakaria EzzoukhryAntoine Galmiche
May 23, 2006·Biochemical and Biophysical Research Communications·Ai-Guo WangNam-Soon Kim
Feb 21, 2004·Biochemical and Biophysical Research Communications·Eunyoung Chun, Ki-Young Lee
Aug 1, 2006·Biochemical and Biophysical Research Communications·Hong Thanh PhamJin-Hyun Ahn
Feb 5, 2008·Biochemical and Biophysical Research Communications·Sun KimJong-Keuk Lee

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