PMID: 9151422Jan 1, 1997

Characterization of cellulase complex of Streptomyces albaduncus

Journal of Basic Microbiology
R K Harchand, S Singh

Abstract

Exoglucanase and endoglucanase (glucanases) enzymes of S. albaduncus were found to be very stable, showing only 36% and 8% loss in activities respectively after 3 days of incubation at 50 degrees C. In contrast, beta-glucosidase was significantly less stable retaining only 22.30% activity after 30 min incubation at 40 degrees C. The glucanases manifested maximum stability in pH range of 5.5-6.0 whereas beta-glucosidase was completely stable over a broad pH range of 6.5-9.0. Both glucanases were enhanced by some cations whereas beta-glucosidase did not require any cation for activity. K(m) values for crude exoglucanase, endoglucanase and beta-glucosidase were 40.00 mg/ml, 92.30 mg/ml and 1.714 mM with maximum reaction velocities (Vmax) of 0.606, 33.330 and 0.109 IU/mg of protein, respectively. The enzymes were subject to end-product inhibition, with exo- and endo-glucanases decreasing by 20% and 70% respectively, in the presence of 0.3% glucose. However, beta-glucosidase showed marked resistance to glucose inhibition, retaining 59% of residual activity even in the presence of 30% glucose in the reaction mixture. This characteristic may be advantageous in the commercial exploitation of enzyme system. An activation of beta-glucosi...Continue Reading

References

Jan 1, 1979·Folia Microbiologica·J K Gupta, Y P Gupta
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Jan 1, 1990·The Journal of Applied Bacteriology·P S BaggaS Sharma
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Citations

Jul 20, 2010·Interdisciplinary Sciences, Computational Life Sciences·Jiji GeorgeGurusamy Annadurai
Apr 27, 2002·Applied and Environmental Microbiology·Ranjeet K TokalaM J Morra
Jan 23, 2016·Enzyme Research·Anita SainiAnita Yadav

Related Concepts

Emulsion beta-D-Glucosidase
Cations
Endoglucanase Y
Edetic Acid, Sodium Salt
Glucose, (beta-D)-Isomer
Heating
Hydrogen-Ion Concentration
Streptomyces
Beta-1,3-Glucanase Type II
Cellobiohydrolase II

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