Abstract
Trichoderma asperellum, a traditional bio-control species, was demonstrated to be an excellent candidate for lignocellulose degradation in this work. Comparing to the representatively industrial strain of Trichoderma reeseiQM6a, T. asperellum T-1 showed more robust growth, stronger spore production, faster secretion of lignocellulose-decomposing enzymes and better pH tolerance. The reducing sugar released by strain T-1 on the second day of fermentation was 87% higher than that of strain QM6a, although the maximum reducing sugar yield and the cellulase production persistence of the strain T-1 were lower. Our experiment found that the cellulase secretion was strongly inhibited by glucose, suggesting the existence of carbon source repression pathway in T. asperellum T-1. The inhibiting effect was enhanced with an increase in glucose concentration and was closely related to mycelium growth. SDS-PAGE and secondary mass-spectrum identification confirmed that the expression of endo-1,4-β-xylanase I in T. asperellum T-1 was down-regulated when glucose was added. The factor Cre1, which plays an important role in the down-regulation of the endo-1,4-β-xylanase I gene, was investigated by bioinformatics methods. The protein structure of Cr...Continue Reading
References
Nov 1, 1991·Molecular and Cellular Biology·C E Dowzer, J M Kelly
Aug 1, 1990·Molecular and Cellular Biology·A SakaiF Hishinuma
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S el-GogaryH el-Dorry
Nov 27, 1995·FEBS Letters·J StraussC P Kubicek
Jun 24, 1996·Molecular & General Genetics : MGG·M IlménM Penttilä
Jan 1, 1996·Bioscience, Biotechnology, and Biochemistry·S TakashimaT Uozumi
Feb 11, 1999·Bioscience, Biotechnology, and Biochemistry·S TakashimaT Uozumi
Jul 14, 1999·FEBS Letters·G VautardM Fèvre
Feb 13, 2001·Journal of Bacteriology·M OrejasD Ramón
Jan 7, 2003·Applied and Environmental Microbiology·Nina AroMerja Penttilä
Aug 29, 2003·Current Opinion in Biotechnology·Joel R Cherry, Ana L Fidantsef
Feb 10, 2007·Science·Michael E HimmelThomas D Foust
Dec 8, 2007·Fungal Genetics and Biology : FG & B·Preeti RoyJoan M Kelly
Sep 23, 2008·Microbiological Research·César Marcos MarcelloCirano José Ulhoa
Oct 28, 2008·Bioresource Technology·Mahesh ChandraRajinder S Sangwan
Nov 17, 2009·Molecular Microbiology·Wilfried Jonkers, Martijn Rep
Sep 20, 2011·Bioresource Technology·Hui LinYuhua Zhao
Feb 4, 2012·BMC Genomics·Marie CouturierJean-Guy Berrin
Feb 23, 2012·Molecular & Cellular Proteomics : MCP·Sunil S AdavSiu Kwan Sze
Nov 28, 2012·Science·Shi-You DingEdward A Bayer
May 15, 2013·Applied Biochemistry and Biotechnology·M F IbrahimS Abd-Aziz
Nov 30, 2013·Biotechnology for Biofuels·Isa Jacoba MarxHeinrich Volschenk
Jul 24, 2015·Environmental Science. Processes & Impacts·A HolbachS Norra
Citations
Sep 24, 2016·World Journal of Microbiology & Biotechnology·Hiroyuki InoueShigeki Sawayama
Aug 6, 2018·Applied Microbiology and Biotechnology·Scott E Baker
Jun 21, 2018·MBio·Leandro José de AssisGustavo Henrique Goldman