Characterization of chemically tritiated microcystin-LR and its distribution in mice

Toxicon : Official Journal of the International Society on Toxinology
N A RobinsonJ G Pace

Abstract

Chemically tritiated microcystin-LR (spec. act. 194 mCi/mmol), purified to greater than 95% by C-18 reverse-phase high performance liquid chromatography, exhibited the same retention time and ultraviolet absorption profile as unlabeled toxin. Acid-hydrolyzed [3H]-toxin yielded tritiated glutamate and beta-methylasparate. Stability of the nonexchangeable [3H]-toxin in saline and urine was greater than 93% after 42 days stored at 22 degrees, 4 degrees or -20 degrees C. In blood, the breakdown of toxin was temperature- and time-dependent (63% at 22 degrees C, 28 days). Unlabeled toxin was stable for greater than 42 days stored at either 4 degrees or -20 degrees C in saline. The LD50 (mouse, i.p.) of [3H]-microcystin-LR and unlabeled toxin was the same [75 micrograms/kg (65-90) and 65 micrograms/kg (53-80), respectively]. From 3 to 90 min after i.p. injection of 70 micrograms/kg [3H]-microcystin-LR there was a slow absorption of toxin from the peritoneal cavity and efficient accumulation in liver. The elimination half-life of the plasma concentration curve was 29 min. Tritium distribution in tissue at death or 6 hr post injection was similar for all doses (13-101 micrograms/kg). At 101 micrograms/kg, liver contained 56 +/- 1%, inte...Continue Reading

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Citations

Jan 1, 1991·Journal of Biochemical Toxicology·N A RobinsonJ G Pace
Jan 31, 2012·Fish Physiology and Biochemistry·Wenshan ZhouXuezhen Zhang
Jun 11, 1990·Biochimica Et Biophysica Acta·J E ErikssonJ A Meriluoto
Jan 1, 1990·Toxicon : Official Journal of the International Society on Toxinology·J A MeriluotoJ E Eriksson
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·M T RunnegarI R Falconer
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·S B HooserW M Haschek
Jan 1, 1991·Toxicon : Official Journal of the International Society on Toxinology·G A MiuraJ G Pace
Feb 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·D E WilliamsC F Holmes
Nov 1, 1992·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W L Thompson, J G Pace
Dec 12, 2001·Toxicon : Official Journal of the International Society on Toxinology·Yongding LiuTongming Liu
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Lisa SpoofJussi Meriluoto
Oct 16, 2003·Toxicon : Official Journal of the International Society on Toxinology·Aleksandra MilutinovićDusan Suput
Jun 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·R R StottsV R Beasley
Aug 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·R M Dawson
Jun 10, 1998·Toxicon : Official Journal of the International Society on Toxinology·T H LeeH N Chou
Jun 5, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Christelle MalbrouckPatrick Kestemont
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Apr 13, 2010·Mutation Research·Lilianne Abramsson-ZetterbergRoland Mattsson
Jul 2, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yanyan ZhaoDapeng Li
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