PMID: 7543213Jul 1, 1995Paper

Characterization of chicken CD8-specific monoclonal antibodies recognizing novel epitopes

Scandinavian Journal of Immunology
M LuhtalaO Vainio

Abstract

CD8 is a heterodimeric cell surface glycoprotein expressed primarily on thymocytes and a subpopulation of mature T lymphocytes. It binds to the invariant part of the major histocompatibility complex class I molecule and participates in antigen recognition by the major histocompatibility complex class I restricted T cells. As in mammalian species, the majority of chicken thymocytes express both CD4 and CD8, whereas peripheral T cells are either CD4- or CD8-positive. We have created a panel of mouse monoclonal antibodies detecting different cell surface epitopes on chicken CD8. The antibodies precipitate a 32-34 kDa dimeric protein from surface labelled thymocytes under reducing conditions. The identical N-deglycosylation pattern confirms that these MoAb precipitate the same heterodimeric molecule from chicken thymocyte lysates. Binding of 11-38 and 11-39 MoAb to peripheral blood T cells is totally inhibited by 11-39 and previously characterized CT8 and EP72 MoAb, further confirming their CD8 specificity. CD8 alpha-chain specificity of MoAb 11-39, 11-38, 11-30 and 11-13 is conclusively proven by staining COS-cells transfected with a plasmid containing CD8 alpha cDNA. However, MoAb 11-13, 11-30 and 11-38 do not compete with MoAb 1...Continue Reading

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Citations

Sep 12, 2000·Developmental and Comparative Immunology·S LawsonP Kaiser
Sep 13, 2003·Veterinary Immunology and Immunopathology·Yongqiang WangOlli Vainio
Mar 31, 1998·Scandinavian Journal of Immunology·M LampisuoO Lassila
Mar 17, 2007·Avian Pathology : Journal of the W.V.P.A·Dana PrůkováJan Svoboda
Dec 1, 1996·The Journal of Cell Biology·O VainioB A Imhof
Feb 18, 2016·Applied Biochemistry and Biotechnology·Beibei ChengAnchun Cheng
Apr 1, 2008·Developmental and Comparative Immunology·Sonja KothlowJohn R Young
Dec 20, 2005·Developmental and Comparative Immunology·Ryan L BohlsEllen W Collisson
Feb 11, 2003·The Journal of Veterinary Medical Science·Maki MotobuYoshikazu Hirota
May 8, 2013·Developmental and Comparative Immunology·Birgit C ViertlboeckThomas W Göbel
Feb 23, 2010·Molecular Immunology·Petar PetrovOlli Vainio
Oct 20, 2018·Proceedings of the National Academy of Sciences of the United States of America·Luca D BertzbachBenedikt B Kaufer
Dec 15, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tania MarchbankRaymond J Playford
May 3, 2018·Frontiers in Immunology·Franziska RohdeBernd Kaspers
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·B A ImhofO Vainio
Aug 7, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas W GöbelBernd Kaspers

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