Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR.

Journal of Veterinary Science
Khalid Munir

Abstract

We have previously described the development of a onetube SYBR Green real-time RT-PCR assay for the detection and quantitation of infectious salmon anemia virus (ISAV) in various biological samples. The twofold aim of the present study was to verify that the optimized SYBR Green real-time RT-PCR conditions could detect ISAV isolates of different geographic origins, and to analyze the growth patterns of the selected ISAV isolates in the Chinook head salmon embryo (CHSE) -214 cells by this assay to better characterize their CHSE-phenotypes. A total of 24 ISAV isolates were used in this study. The results indicated that the SYBR Green real-time RT-PCR could detect ISAV of different geographic origins or laboratory sources. The capacity of ISAV isolates to cause cytopathic effects (CPE) in the CHSE-214 cell line, viral titration of the infected CHSE-cell harvests, and analysis of viral RNA levels in CHSE-214 cells at post-infection day zero, 7 and 14 by SYBR Green real-time RT-PCR confirmed the existence of three CHSE-phenotypes of ISAV: replicating cytopathic, replicating non-cytopathic, and non-replicating non-cytopathic. The identification of these three CHSE- phenotypes of ISAV has important implications from diagnostic and bio...Continue Reading

References

Mar 27, 1999·Diseases of Aquatic Organisms·D BouchardB L Nicholson
Jun 1, 2001·Diseases of Aquatic Organisms·H I Wergeland, R A Jakobsen
Jun 20, 2001·Diseases of Aquatic Organisms·F S KibengeD Wadowska
Jun 20, 2001·The Journal of General Virology·B KrossøyE Biering
Oct 27, 2001·Diseases of Aquatic Organisms·R S RaynardA Gregory
Jan 25, 2002·The Journal of General Virology·Sharon C ClouthierEric D Anderson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·H G Niesters
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·C T WittwerK S Elenitoba-Johnson
Mar 9, 2002·Nucleic Acids Research·Ian M MackayAndreas Nitsche
Jun 22, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Espen Rimstad, Siri Mjaaland
Jun 25, 2002·Diseases of Aquatic Organisms·Trond Løvdal, Oivind Enger
Aug 16, 2002·Diseases of Aquatic Organisms·A Gregory
Oct 22, 2002·The Journal of General Virology·Marianne KristiansenTor Gjøen
Dec 7, 2002·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Hubert G M Niesters
May 29, 2003·Biologicals : Journal of the International Association of Biological Standardization·Julia F Ridpath
Jun 18, 2003·The Journal of General Virology·Tomy JosephFrederick S B Kibenge
Oct 11, 2003·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Emeka E MonekeFrederick S B Kibenge
Oct 25, 2003·Journal of Fish Diseases·J B Rolland, J R Winton
Mar 17, 2004·Journal of Virological Methods·Khalid Munir, Frederick S B Kibenge
Oct 6, 2004·Animal Health Research Reviews·Frederick S B KibengeEmeka Moneke
Apr 14, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jill B RollandJames R Winton
Sep 24, 2005·Diseases of Aquatic Organisms·H PlarreA Nylund
Oct 6, 2005·Journal of Virological Methods·Kjartan Hodneland, Curt Endresen

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Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Minitab
LightCycler

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