Characterization of Chloroethylene Dehalogenation by Cell Extracts of Desulfomonile tiedjei and Its Relationship to Chlorobenzoate Dehalogenation.

Applied and Environmental Microbiology
G T Townsend, J M Suflita

Abstract

We characterized the reductive dehalogenation of tetrachloroethylene in cell extracts of Desulfomonile tiedjei and compared it with this organism's 3-chlorobenzoate dehalogenation activity. Tetrachloroethylene was sequentially dehalogenated to trichloro- and dichloroethylene; there was no evidence for dichloroethylene dehalogenation. Like the previously characterized 3-chlorobenzoate dehalogenation activity, tetrachloroethylene dehalogenation was heat sensitive, not oxygen labile, and increased in proportion to the amount of protein in assay mixtures. In addition, both dehalogenation activities were dependent on hydrogen or formate as an electron donor and had an absolute requirement for either methyl viologen or triquat as an electron carrier in vitro. Both activities appear to be catalyzed by integral membrane proteins with similar solubilization characteristics. Dehalogenation of tetrachloroethylene was inhibited by 3-chlorobenzoate but not by the structural isomers 2- and 4-chlorobenzoate. The last two compounds are not substrates for D. tiedjei. These findings lead us to suggest that the dehalogenation of tetrachloroethylene in D. tiedjei is catalyzed by a dehalogenase previously thought to be specific for meta-halobenzoates.

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Citations

Oct 1, 2002·FEMS Microbiology Ecology·Young-Cheol ChoG-Yull Rhee
Oct 31, 1998·Applied and Environmental Microbiology·D DennieR Beaudet
Jun 1, 1997·Current Opinion in Biotechnology·G Wohlfarth, G Diekert
Oct 1, 1996·Applied and Environmental Microbiology·F E LofflerJ M Tiedje
Feb 1, 1997·Applied and Environmental Microbiology·S el FantroussiS N Agathos
Sep 18, 1997·Applied and Environmental Microbiology·G T Townsend, J M Suflita
Mar 15, 2006·Applied and Environmental Microbiology·B M RosnerA M Spormann

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