PMID: 9428686Jan 15, 1998Paper

Characterization of cholesterol oxidase from Streptomyces hygroscopicus and Brevibacterium sterolicum

European Journal of Biochemistry
G GaddaS Ghisla

Abstract

The FAD-containing enzyme cholesterol oxidase catalyzes the oxidation and isomerization of 3beta-hydroxysteroids having a trans double bond at delta5-delta6 of the steroid ring backbone to the corresponding delta4-3-ketosteroid. Two representative enzymes of this family, namely cholesterol oxidase from Streptomyces hygroscopicus (SCO) and the recombinant enzyme from Brevibacterium sterolicum (BCO) expressed in Escherichia coli, have been characterized herein in their chemical, physical, and biochemical properties. In the native form, both enzymes are monomeric (55 kDa), acidic (pI 4.4-5.1) and contain oxidized FAD (peaks in the 370-390-nm and 440-470-nm regions). Marked differences exist between the oxidized, reduced, and (red) anion semiquinone spectra of the two enzymes, suggesting substantial differences in the flavin microenvironment. Both enzymes form reversibly flavin N(5)-sulfite adducts via measurable k(on) and k(off) steps. BCO has a higher affinity for sulfite (Kd approximately 0.14 mM) compared to SCO (approximately 24 mM). This correlates well with the midpoint redox potentials of the bound flavin, which in the case of BCO are about 100 mV more positive than for SCO. Both enzymes show a high pKa (approximately 11.0)...Continue Reading

References

Jan 10, 1978·Biochemistry·V MasseyP Hemmerich
Jan 1, 1989·Journal of Bacteriology·T IshizakiY Murooka
Jun 1, 1980·Biochemical Society Transactions·V Massey, P Hemmerich
Dec 1, 1994·Applied and Environmental Microbiology·D R CorbinJ P Purcell
Nov 15, 1993·Biochemical and Biophysical Research Communications·J P PurcellR D Sammons
Jun 1, 1953·The Biochemical Journal·L G WHITBY

❮ Previous
Next ❯

Citations

Apr 2, 2004·Biochimica Et Biophysica Acta·Robert M G HynsonRona R Ramsay
May 24, 2000·The Journal of Steroid Biochemistry and Molecular Biology·J MacLachlanC J Brooks
Nov 28, 2007·Journal of Bacteriology·Yin-Ru ChiangGeorg Fuchs
Mar 4, 2009·Biochimie·Mattia PedottiLoredano Pollegioni
May 15, 2009·The FEBS Journal·Dominic P H M HeutsMarco W Fraaije
Jan 26, 2008·Protein Science : a Publication of the Protein Society·Laura CaldinelliLoredano Pollegioni
Oct 22, 2009·The FEBS Journal·Loredano PollegioniGianluca Molla
Oct 22, 2009·The FEBS Journal·Alice Vrielink, Sandro Ghisla
Jul 15, 2015·Applied Microbiology and Biotechnology·Mathias PicklKurt Faber
Jan 5, 2013·Toxicology·Xavier TekpliDominique Lagadic-Gossmann
Apr 10, 2017·The Journal of Biological Chemistry·Konstantin G ChernovVladislav V Verkhusha
Mar 10, 2007·Prikladnaia biokhimiia i mikrobiologiia·M V Donova
Feb 12, 2008·Journal of Cellular Physiology·Xavier TekpliDominique Lagadic-Gossmann
Apr 9, 2005·The Journal of Biological Chemistry·Laura CaldinelliLoredano Pollegioni
Jul 18, 2008·Molecular BioSystems·Jesús F Aparicio, Juan F Martín
Dec 10, 1999·The Journal of Biological Chemistry·M W FraaijeA Mattevi
Feb 20, 2007·The Journal of Biological Chemistry·Yin-Ru ChiangGeorg Fuchs
Jul 22, 2006·The Biochemical Journal·Louis LimAlice Vrielink
Jul 21, 2004·The Journal of Steroid Biochemistry and Molecular Biology·V M NikolayevaM V Donova
Apr 22, 2006·The Journal of Organic Chemistry·Sabita Patel, B K Mishra
Sep 17, 2003·Accounts of Chemical Research·Nicole S Sampson, Alice Vrielink

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.