PMID: 9420265Jan 7, 1998Paper

Characterization of cis-acting and NS1 protein-responsive elements in the p6 promoter of parvovirus B19

Journal of Virology
R GareusSusanne Modrow

Abstract

Parvovirus B19 infections are associated with diverse clinical manifestations, ranging from no symptoms to severe symptoms. The virus shows an extreme tropism for replication in erythroid progenitor cells, possibly due to the activity of the only functional promoter (p6) of the B19 virus genome in combination with both cell- and cell cycle-specific factors and the trans-activator protein NS1. As presented here, p6 promoter sequences derived from several B19 virus isolates proved to be highly conserved. Furthermore, mutations did not affect any of the potential binding sites for transcription factors. One variation of the base at position 223 was identified only in B19 virus isolates derived from patients with persistent infection or chronic arthritis. To determine promoter activity and to characterize regulatory elements, sequences spanning the total p6 promoter and subfragments of them were introduced into a eukaryotic expression vector upstream of the luciferase gene (from Photinus pyralis). After transfection into HeLa, CEM, BJAB, and K562 cells, the p6 promoter was found to be highly active. When introduced into the erythroid cell line K562, p6-controlled transcription exceeded that of the simian virus 40 promoter-enhancer ...Continue Reading

References

Aug 1, 1987·Journal of Virology·K OzawaN Young
Apr 1, 1987·Virology·M C BlundellC R Astell
Jan 1, 1984·The Journal of Clinical Investigation·N S YoungR K Humphries
Mar 1, 1995·The Journal of General Virology·A von PoblotzkiS Modrow
Oct 1, 1993·Science·K E BrownN S Young
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Mader, J H White
Aug 1, 1996·The Journal of General Virology·A HemauerS Modrow

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Citations

Feb 19, 2011·Future Virology·Aaron Yun Chen, Jianming Qiu
Nov 4, 2016·Clinical Microbiology Reviews·Jianming QiuNeal S Young
Aug 21, 2002·Journal of Virology·Annabelle ServantAntoine Garbarg-Chenon
Apr 6, 2007·Journal of Virology·Anna EkmanMaria Söderlund-Venermo
May 23, 2001·The Journal of General Virology·U RaabS Modrow
Oct 29, 2005·Journal of Virology·Johannes BlümelJohannes Löwer
Jun 10, 2003·Arthritis and Rheumatism·Hartwig W LehmannSusanne Modrow
Mar 1, 2013·Future Virology·Yong Luo, Jianming Qiu
Jun 10, 2008·Intervirology·Juha Lindner, Susanne Modrow
Jun 21, 2018·Frontiers in Cellular and Infection Microbiology·Safder S Ganaie, Jianming Qiu
Sep 6, 2001·Journal of Medical Virology·K ZakrzewskaG Tagariello
Jul 16, 2002·Pathologie-biologie·S Pillet, F Morinet
Aug 26, 2004·Virology·Sylvie PilletFrédéric Morinet
Sep 30, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Paola FrancalanciNeil E Bowles
Nov 3, 2004·Virology·Akitoshi NakashimaKazuo Sugamura
Nov 5, 2016·Biochemistry·Jonathan L SanchezNancy C Horton
Jun 2, 2009·Rheumatic Diseases Clinics of North America·Ines Colmegna, Noah Alberts-Grill
Aug 15, 2017·Virology·Tyler JanovitzErik Falck-Pedersen

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