Characterization of cis-acting elements affecting strength and phloem specificity of the coconut foliar decay virus promoter

The Journal of General Virology
A Hehn, W Rohde

Abstract

During replication in its host plant, coconut foliar decay virus (CFDV) remains restricted to the phloem tissue. Previous in vivo studies on subgenomic CFDV DNA had provided evidence for the phloem specificity of the CFDV promoter. Here, new promoter constructs are described which are distinguished by the presence or absence of various cis-acting signals and which gave rise to a 16-fold higher reporter gene (beta-glucuronidase) activity (reaching 30% of the cauliflower mosaic virus 35S promoter) in tobacco protoplasts, while the phloem specificity in transgenic tobacco plants was conserved. Surprisingly, the CFDV stem-loop structure dramatically influenced transcriptional efficiency. From these studies and sequence comparisons with other phloem-specific promoters, cis-signals involved in CFDV promoter strength and tissue specificity were identified.

Citations

Sep 20, 2005·Virus Research·Cuiping Guan, Xueping Zhou
Feb 26, 2005·Virology Journal·Jeff VeltenChristopher I Cazzonelli
Jan 6, 2004·Proceedings of the National Academy of Sciences of the United States of America·Shunhong DaiRoger N Beachy
Aug 19, 2007·Plant Molecular Biology·Gundula A NollDirk Prüfer
Jun 1, 2010·Indian Journal of Virology : an Official Organ of Indian Virological Society·Bikash Mandal
Jan 28, 2005·Transgenic Research·Hongnian GuoYingchuan Tian

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