Characterization of clostridia by gas chromatography. I. Differentiation of species by cellular fatty acids.

Applied Microbiology
C W Moss, V J Lewis

Abstract

Fatty acids of 41 strains representing 13 species of Clostridium were extracted directly from whole cells and examined as methyl esters by gas-liquid chromatography. Both visual and quantitative comparisons of the resulting chromatograms for the presence and relative amounts of large major peaks allowed rapid differentiation of C. perfringens, C. sporogenes, and C. bifermentans from each other and from 10 other species. Each of the three former species possessed a different characteristic fatty acid methyl ester profile that was exhibited by all strains tested within the respective species. Culture age and growth media influenced the relative proportions of certain of the acids, but such differences did not limit species differentiation.

References

Jul 1, 1966·Applied Microbiology·Y HenisM Alexander
Jun 1, 1966·Journal of Dairy Science·R E Bawdon, R Bassette
Aug 1, 1964·Journal of Bacteriology·J A CROOMS B TOVE
Dec 1, 1962·Journal of Bacteriology·A G Marr, J L Ingraham

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Citations

Oct 1, 1970·Journal of Bacteriology·C W MossJ D Thayer
Jul 1, 1972·Journal of Bacteriology·L L KostiwB J Tyson
Jun 1, 1973·Journal of Bacteriology·C W MossR M McKinney
Feb 1, 1970·Applied Microbiology·C W MossD J Goldsmith
Apr 1, 1971·Applied Microbiology·C O ThoenR D Ellefson
Oct 1, 1972·Applied Microbiology·C W MossR E Weaver
Oct 1, 1969·Journal of Bacteriology·C W Moss, W E Dunkelberg
Oct 1, 1991·Clinical Microbiology Reviews·D F Welch
Aug 1, 1989·Applied and Environmental Microbiology·Y SakagamiT Tashima
Feb 1, 1969·Journal of Bacteriology·C W MossW B Cherry
Nov 1, 1975·Journal of Bacteriology·I J Sud, D S Feingold
Feb 1, 1977·Applied and Environmental Microbiology·D B Drucker, F J Veazey
Feb 1, 1970·Journal of Bacteriology·J G Voss
Oct 1, 1979·Antimicrobial Agents and Chemotherapy·I J Sud, D S Feingold
Apr 19, 1968·Science·B M MitrukaL E Carmichael

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