Characterization of collagen fibrils after equine suspensory ligament injury: an ultrastructural and biochemical approach

The Veterinary Journal
M K Shikh AlsookE Baise

Abstract

Suspensory ligament (SL) injuries are an important cause of lameness in horses. The mechanical properties of connective tissue in normal and pathological ligaments are mainly related to fibril morphology, as well as collagen content and types. The purpose of this study was to evaluate, using biochemical and ultrastructural approaches, the alterations in collagen fibrils after injury. Eight Warmblood horses with visible signs of injury in only one forelimb SL were selected and specimens were examined by transmission electron microscope (TEM). Collagen types I, III and V were purified by differential salt precipitation after collagen extraction with acetic acid containing pepsin. TEM revealed abnormal organization as well as alterations in the diameter and shape of fibrils after SL injury. The bands corresponding to types I, III and V collagen were assessed by densitometry after sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Densitometric analysis indicated that the proportions of type III and type V collagen were higher (P < 0.001) in damaged tissues compared with normal tissues with a mean increase of 20.9% and 17.3%, respectively. Concurrently, a decrease (P < 0.001) in type I collagen within damaged tis...Continue Reading

References

Dec 18, 1978·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D A ParryG R Barnes
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R FleischmajerP N Graves
Mar 1, 1993·Pathology, Research and Practice·L JózsaP Kannus
Oct 3, 1999·Equine Veterinary Journal·H L BirchA E Goodship
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·D E Birk
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·B Eyden, M Tzaphlidou
Sep 28, 2000·Micron : the International Research and Review Journal for Microscopy·K MizunoT Hayashi
Nov 4, 2000·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·C NiyibiziS L Woo
Jan 5, 2002·American Journal of Veterinary Research·W CherdchuthamA Barneveld
May 25, 2002·Joint, Bone, Spine : Revue Du Rhumatisme·Joaquim Gonçalves-NetoH H Yoshinari
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Heidi A EriksenJuha Risteli
Apr 2, 2003·Biophysical Journal·Thomas GutsmannPaul K Hansma
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Z DeylA Eckhardt
Jun 17, 2004·Molecular and Cellular Biology·Hélène Chanut-DelalandeFlorence Ruggiero
Sep 24, 2004·The Journal of Biological Chemistry·Richard J WenstrupDavid E Birk
Jul 23, 2005·IUBMB Life·Arnab Bhattacharjee, Manju Bansal
Jul 22, 2006·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Karin Grävare SilbernagelJon Karlsson
Jan 6, 2007·The Veterinary Journal·Ellen R SingerJane K Murray
Dec 14, 2007·Connective Tissue Research·Takafumi WatanabeKazushige Takehana
Jun 30, 2011·PloS One·Ping LuHong Wei Ouyang
Sep 14, 2011·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Connie S ChamberlainRay Vanderby
Sep 20, 2011·Journal of Hand Therapy : Official Journal of the American Society of Hand Therapists·James H-C WangBin Li
Feb 7, 2012·Cell Stem Cell·Olle LindvallClive N Svendsen
Mar 27, 2012·Stem Cells International·Mark Young
Nov 28, 2012·The Veterinary Clinics of North America. Equine Practice·Natasha M Werpy, Jean-Marie Denoix
Jul 26, 2013·Muscles, Ligaments and Tendons Journal·Simone VesentiniAlfonso Gautieri
Nov 28, 2013·American Journal of Veterinary Research·Megan R WilliamsJohn A Stick

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