PMID: 8595862Jan 15, 1996Paper

Characterization of conjugative transposon Tn5251 of Streptococcus pneumoniae

FEMS Microbiology Letters
R ProvvediG Pozzi

Abstract

Tn5251 belongs to the Tn916-Tn1545 family of conjugative transposons (CT) and was found integrated into CT Tn5252, to form the composite element Tn5253 of Streptococcus pneumoniae. We show that Tn5251 is identical in structure and size to Tn916. DNA sequence analysis of a 4,419-bp segment containing the tet(M) gene showed that only 73 nucleotides out of 4,419 were different in the two CT. Essentially all differences (66/73) were clustered in a 688-bp segment of tet(M), which was 90% identical to Tn916 and 100% identical to the tet(M) genes of Tn1545 from S. pneumoniae and pOZ101 from Neisseria gonorrhoeae. DNA sequence analysis of the Tn5251/Tn5252 junction fragments allowed us (i) to determine Tn5251 termini, (ii) to define the 6-bp coupling sequences flanking the CT, and (iii) to infer the structure of the integration site (attB) of Tn5251 into Tn5252. Conjugal transfer of Tn5251 independent from Tn5253 could not be detected, even if we could show excision and formation of Tn5251 circular intermediates at a level of 5.4 copies per 10(6) chromosomes.

References

Aug 1, 1976·Journal of Virology·R D Porter, W R Guild
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1992·Antimicrobial Agents and Chemotherapy·F BentorchaT Horaud
Oct 1, 1992·Clinical Microbiology Reviews·B S SpeerA A Salyers
Jan 3, 1991·Nature·J M SmithB G Spratt
Nov 1, 1989·Journal of General Microbiology·H M FletcherL Daneo-Moore
Jun 1, 1986·Journal of Bacteriology·M N VijayakumarW R Guild
Jun 1, 1986·Journal of Bacteriology·M N VijayakumarW R Guild
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Oct 1, 1980·Journal of Bacteriology·M D Smith, W R Guild
Jun 1, 1995·Trends in Microbiology·D B ClewellD D Jaworski
Jun 1, 1994·Journal of Bacteriology·C K Rudy, J R Scott

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Citations

Jul 10, 2001·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·J DopazoJ F García-Bustos
Apr 9, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·C A Widdowson, K P Klugman
Oct 19, 2000·Antimicrobial Agents and Chemotherapy·N DohertyC G Dowson
May 25, 2004·Antimicrobial Agents and Chemotherapy·Maria Del GrossoAnnalisa Pantosti
Jan 11, 2007·Antimicrobial Agents and Chemotherapy·Radosław IzdebskiWaleria Hryniewicz
Apr 24, 2015·Computational and Structural Biotechnology Journal·Chrispin ChaguzaDean B Everett
Oct 9, 2003·Journal of Medical Microbiology·Ralf René ReinertRudolf Lütticken
Jun 11, 2011·FEMS Microbiology Reviews·Adam P Roberts, Peter Mullany
Aug 16, 2018·Frontiers in Microbiology·Francesco SantoroFrancesco Iannelli
Dec 4, 2013·Antimicrobial Agents and Chemotherapy·Francesco IannelliGianni Pozzi
Jan 26, 2011·Antimicrobial Agents and Chemotherapy·Marina MingoiaMaria Pia Montanari
Apr 28, 2009·FEMS Microbiology Reviews·María Pilar Garcillán-BarciaFernando de la Cruz
Apr 1, 1997·Journal of Bacteriology·A R Sánchez-BeatoJ L García

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