PMID: 9166725Jun 1, 1997Paper

Characterization of CPP32-like protease activity following apoptotic challenge in SH-SY5Y neuroblastoma cells

Journal of Neurochemistry
R PosmanturK K Wang

Abstract

We characterized the activation of interleukin-1beta-converting enzyme (ICE)-like proteases (caspases) in human neuroblastoma cells (SH-SY5Y) following challenge with staurosporine, an established agent known to induce apoptosis. Time course analyses of lactate dehydrogenase release detected a significant increase in cell death as early as 6 h that continued at least until 24 h following staurosporine treatment. Western blot analyses using anti-poly(ADP-ribose) polymerase (anti-PARP) and anti-CPP32 antibodies revealed proteolytic processing of CPP32 (an ICE homologue) as well as fragmentation of PARP as early as 3 h following staurosporine challenge. Furthermore, the hydrolysis of the CPP32 substrate acetyl-DEVD-7-amido-4-methylcoumarin was detected as early as 3 h and became maximal at 6 h after staurosporine challenge, suggesting a delayed and sustained period of CPP32-like activation. In addition, we used the first immunohistochemical examination of CPP32 and PARP in cells following an apoptotic challenge. The localization of CPP32 in untreated SH-SY5Y cells was exclusively restricted to the cytoplasm. Following staurosporine challenge there was a condensing of CPP32 immunofluorescence from the cytoplasm to a region adjacent...Continue Reading

Citations

Aug 26, 1998·The Journal of Biological Chemistry·K K WangJ S Morrow
Feb 26, 1999·Journal of Neurochemistry·B R ChakravarthyJ P MacManus
May 18, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J P MacManusU Tuor
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Aug 6, 2000·British Journal of Pharmacology·J JordánV Ceña
Jul 5, 2002·The Journal of Nutrition·Carmel Avivi-GreenBetty Schwartz
Jan 1, 2008·Journal of Biomedical Optics·Manuel KemmlerChristian Hoffmann
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chrysanthi SamaraMehmet Fatih Yanik
Apr 25, 2001·Journal of Neuroscience Research·A Valencia, J Morán
Aug 10, 2001·The Journal of Biological Chemistry·G N Bijur, R S Jope
Mar 23, 1999·The Journal of Cell Biology·J F KrebsL C Fritz
Apr 2, 1999·The European Journal of Neuroscience·A M GormanS Ceccatelli
Dec 10, 1999·Journal of Neurobiology·C L DelaneyE L Feldman
Apr 1, 2005·Journal of Biomolecular Screening·Martha A O'BrienKeith V Wood
May 17, 2006·Apoptosis : an International Journal on Programmed Cell Death·Shankar SadasivanKevin K W Wang
May 5, 2006·Journal of Neuroscience Research·Chunnian ZhaoPeter P Li
Apr 17, 2003·Experimental Brain Research·Michael J HaasArshag D Mooradian
Jun 18, 2002·Journal of Neurochemistry·Janusz Tucholski, Gail V W Johnson
Oct 27, 2001·The Journal of Biological Chemistry·X BianA W Opipari
Jul 13, 2000·Cell Death and Differentiation·C M van GolenE L Feldman
Dec 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Clay W ScottDavid Aharony
Nov 24, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M Maris, K K Matthay
Nov 22, 2000·The Journal of Cell Biology·L Faleiro, Y Lazebnik
Feb 19, 2000·Journal of Cellular Biochemistry·C Avivi-GreenB Schwartz
Jan 25, 2007·British Journal of Pharmacology·I PosadasV Ceña
Mar 19, 1999·Journal of Neuroscience Research·O Bar-PeledJ D Rothstein
Oct 18, 2000·Toxicology and Applied Pharmacology·K CarlsonM Ehrich
Oct 15, 1998·Experimental Cell Research·R PosmanturR B Gilbertsen
Aug 15, 1998·Archives of Biochemistry and Biophysics·K K WangH Allen

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