Characterization of crystals of the Hjc resolvase from Archaeoglobus fulgidus grown in gel by counter-diffusion

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Christian BiertümpfelClaude Sauter

Abstract

Holliday junction-resolving enzymes are ubiquitous proteins that play a key role in DNA repair and reorganization by homologous recombination. The Holliday junction-cutting enzyme (Hjc) from the archaeon Archaeoglobus fulgidus is a member of this group. The first Hjc crystals were obtained by conventional sparse-matrix screening. They exhibited an unusually elongated unit cell and their X-ray characterization required special care to avoid spot overlaps along the c* axis. The use of an arc appended to the goniometric head allowed proper orientation of plate-like crystals grown in agarose gel by counter-diffusion. Thus, complete diffraction data were collected at 2.7 A resolution using synchrotron radiation. They belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 37.4, c = 271.8 A.

References

Oct 1, 1990·Acta Crystallographica. Section A, Foundations of Crystallography·L A Tong, M G Rossmann
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jun 27, 1997·Journal of Molecular Biology·M F WhiteD M Lilley
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 25, 1999·Acta Crystallographica. Section D, Biological Crystallography·C R KissingerD B Fogel
Aug 15, 2000·The Journal of Biological Chemistry·M KvaratskheliaM F White
May 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·C S BondW N Hunter
Jun 5, 2001·Nature Reviews. Molecular Cell Biology·D M Lilley, M F White
Sep 28, 2002·Acta Crystallographica. Section D, Biological Crystallography·Christian BiertümpfelClaude Sauter
Oct 17, 2002·Extremophiles : Life Under Extreme Conditions·Klaus NeefBörries Kemper
Dec 25, 2002·Acta Crystallographica. Section D, Biological Crystallography·Claire L MiddletonCharles S Bond
Apr 30, 2003·Journal of Structural Biology·Joseph D NgJuan M García-Ruíz
Oct 14, 2004·Nucleic Acids Research·Claire L MiddletonCharles S Bond
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Eric EnnifarDietrich Suck

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Citations

Dec 17, 2009·Progress in Biophysics and Molecular Biology·Bernard LorberRichard Giegé
Dec 2, 2010·HFSP Journal·Richard Giegé, Claude Sauter

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