Characterization of cucumber fermentation spoilage bacteria by enrichment culture and 16S rDNA cloning

Journal of Food Science
Fred BreidtJae Ho Kim

Abstract

Commercial cucumber fermentations are typically carried out in 40000 L fermentation tanks. A secondary fermentation can occur after sugars are consumed that results in the formation of acetic, propionic, and butyric acids, concomitantly with the loss of lactic acid and an increase in pH. Spoilage fermentations can result in significant economic loss for industrial producers. The microbiota that result in spoilage remain incompletely defined. Previous studies have implicated yeasts, lactic acid bacteria, enterobacteriaceae, and Clostridia as having a role in spoilage fermentations. We report that Propionibacterium and Pectinatus isolates from cucumber fermentation spoilage converted lactic acid to propionic acid, increasing pH. The analysis of 16S rDNA cloning libraries confirmed and expanded the knowledge gained from previous studies using classical microbiological methods. Our data show that Gram-negative anaerobic bacteria supersede Gram-positive Fermincutes species after the pH rises from around 3.2 to pH 5, and propionic and butyric acids are produced. Characterization of the spoilage microbiota is an important first step in efforts to prevent cucumber fermentation spoilage. An understanding of the microorganisms that cause...Continue Reading

References

Jul 1, 1976·Applied and Environmental Microbiology·K A ItoJ A Unverferth
Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Jan 3, 2001·Applied and Environmental Microbiology·S J Oude ElferinkF Driehuis
Jun 6, 2003·Trends in Biotechnology·Michaela HolzerRudolf Braun
Apr 1, 1962·Journal of Bacteriology·R L BALDWINR S EMERY
Oct 22, 2008·Applied and Environmental Microbiology·Julia P Galkiewicz, Christina A Kellogg
Dec 20, 2011·Applied and Environmental Microbiology·Wendy FrancoR F McFeeters

Citations

Sep 2, 2015·International Journal of Food Microbiology·Suzanne D Johanningsmeier, R F McFeeters
Sep 18, 2015·Frontiers in Microbiology·Eduardo Medina-Pradas, Francisco Noé Arroyo-López
Nov 26, 2015·Journal of Food Science·Eduardo MedinaMaría Andrea Azcarate-Peril
Nov 3, 2018·Metabolites·Antonio de CastroAlfredo Montaño
Jul 17, 2020·Frontiers in Microbiology·Zhongjing LuFred Breidt
May 21, 2018·International Journal of Food Microbiology·Katheryne V DaughtryRodolphe Barrangou

Related Concepts

Chromium propionate
DNA, Bacterial
Fermentation
Food Contamination
Food Microbiology
Hydrogen-Ion Concentration
Propionic Acids
Propionibacterium
RNA, Ribosomal, 16S
Cucumber (Vegetable)

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