Characterization of Curcumin/Cyclodextrin Polymer Inclusion Complex and Investigation on Its Antioxidant and Antiproliferative Activities

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Jianping ChenYing Liu

Abstract

The aims of this study were to characterize the curcumin/cyclodextrin polymer inclusion complex using X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and UV⁻vis spectroscopy, and to determine the antioxidant activity of this complex by methods of scavenging 2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals assays and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals assays. The inhibitory effect of inclusion complex on A375 cells was also investigated by CCK-8 assay, Annexin-V/PI staining assay, and caspase activity assay. The results showed that the complex exhibited different physicochemical characteristics from that of free curcumin. Moreover, the inclusion complex exhibited novel antioxidant activity by scavenging the ABTS and DPPH free radicals and displayed higher antiproliferative activity on A375 cells. Further investigation revealed that inclusion complex could induce A375 cell apoptosis. These findings suggest that inclusion complex could be developed as a novel natural antioxidant with potential applications in cancer chemoprevention.

References

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Citations

Jul 14, 2019·AAPS PharmSciTech·Karthikeyan Selvaraj, Bong-Kyu Yoo
Mar 12, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danni ZhengYuxiang Bai
Apr 30, 2020·International Journal of Nanomedicine·Heshu Sulaiman RahmanNoorjahan Banu Alitheen
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Layan DahabraMouhamad Khoder
Apr 16, 2021·International Journal of Nanomedicine·Islam M AdelNermeen A Elkasabgy
Jun 3, 2021·Polymers·Adrián MatencioFrancesco Trotta
May 28, 2021·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Islam M AdelNermeen A Elkasabgy

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Methods Mentioned

BETA
X-ray
Assay
Infrared Spectroscopy
flow cytometry

Software Mentioned

SPSS

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis