Characterization of cytokine-induced myeloid-derived suppressor cells from normal human peripheral blood mononuclear cells.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Melissa G LechnerAlan L Epstein

Abstract

Tumor immune tolerance can derive from the recruitment of suppressor cell populations, including myeloid-derived suppressor cells (MDSCs). In cancer patients, increased MDSCs correlate with more aggressive disease and a poor prognosis. Expression of 15 immune factors (TGFbeta, IL-1beta, IL-4, IL-6, IL-10, GM-CSF, M-CSF, IDO, fms-related tyrosine kinase 3 ligand, c-kit ligand, inducible NO synthase, arginase-1, TNF-alpha, cyclo-oxygenase 2, vascular endothelial growth factor [VEGF]) by MDSC-inducing human solid tumor cell lines was evaluated by RT-PCR. Based upon these data, cytokine mixtures were then tested for their ability to generate suppressive CD33(+) cells from healthy donor PBMCs in vitro by measuring their ability to inhibit the proliferation of, and IFN-gamma production by, fresh autologous human T cells after CD3/CD28 stimulation. Induced MDSCs were characterized with respect to their morphology, surface phenotype, and gene expression profile. MDSC-inducing cancer cell lines demonstrated multiple pathways for MDSC generation, including overexpression of IL-6, IL-1beta, cyclo-oxygenase 2, M-CSF, and IDO. CD33(+) cells with potent suppressive capacity were best generated in vitro by GM-CSF and IL-6, and secondarily by ...Continue Reading

References

Mar 19, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Rebecca L Elliott, Gerard C Blobe
Jun 21, 2006·Molecular Immunology·Chauncey J SpoonerRichard C Schwartz
Oct 28, 2006·Nucleic Acids Research·Wenwu CuiKevin Gardner
May 8, 2007·Cancer Research·Pratima SinhaSuzanne Ostrand-Rosenberg
Jul 4, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rebecca E SadunAlan L Epstein
Oct 25, 2007·Nature Reviews. Cancer·Mario P Colombo, Silvia Piconese
Mar 28, 2008·Immunological Reviews·Ilaria MarigoVincenzo Bronte
Apr 17, 2008·Cancer Research·Srinivas Nagaraj, Dmitry I Gabrilovich
Sep 16, 2008·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Yu LiuRongcun Yang
Oct 4, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Je-In YounDmitry I Gabrilovich
Oct 7, 2008·Oncogene·T J Stewart, S I Abrams
Feb 7, 2009·Nature Reviews. Immunology·Dmitry I Gabrilovich, Srinivas Nagaraj
Feb 24, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ingrid E DumitriuChristopher D Gregory
Feb 25, 2009·International Journal of Cancer. Journal International Du Cancer·Xiaoyu XiangHuang-Ge Zhang
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jennifer S KoJames H Finke
Apr 14, 2009·International Immunopharmacology·Moses K DonkorJames E Talmadge
Apr 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Cesar A CorzoDmitry I Gabrilovich
Jul 21, 2009·Current Opinion in Pharmacology·Stefano UgelVincenzo Bronte
Dec 24, 2009·Cancer Research·Minu K SrivastavaSuzanne Ostrand-Rosenberg
Mar 27, 2010·Science·Mitch Leslie

❮ Previous
Next ❯

Citations

Jan 5, 2012·Cancer Immunology, Immunotherapy : CII·Matthew R PorembkaPeter Goedegebuure
Apr 12, 2011·Breast Cancer Research and Treatment·Maria Libera AsciertoMasoud H Manjili
Mar 23, 2012·Nature Reviews. Immunology·Dmitry I GabrilovichVincenzo Bronte
May 12, 2012·Nature Reviews. Immunology·Bruce R BlazarMehrdad Abedi
Mar 20, 2012·Carcinogenesis·Zvi G Fridlender, Steven M Albelda
Feb 9, 2012·Clinical & Developmental Immunology·Theodore S JohnsonBernard L Maria
Feb 23, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rimas J Orentas
Feb 2, 2013·Transplantation Research·Nataša Obermajer, Pawel Kalinski
Jan 24, 2013·Journal of Leukocyte Biology·Chris P VerschoorDawn M E Bowdish
Dec 14, 2011·Immunotherapy·François MartinFrançois Ghiringhelli
May 11, 2011·Immunotherapy·Courtney Nicholas, Gregory B Lesinski
May 20, 2014·The Journal of Clinical Investigation·Yongjun SuiJay A Berzofsky
Feb 15, 2011·Journal of Biomedicine & Biotechnology·Ming-Yi HoWinnie Yang
Jun 15, 2011·Leukemia & Lymphoma·Erica D Warlick, Jeffrey S Miller
Dec 12, 2013·Expert Review of Clinical Immunology·Alka Bhatia, Yashwant Kumar
Oct 16, 2012·Journal of Drug Targeting·Arpit BhargavaPradyumna Kumar Mishra
Feb 3, 2016·Immunology and Cell Biology·Si ChenJinping Zhang
Jan 29, 2016·Journal of Immunology Research·Eileen Uribe-Querol, Carlos Rosales
Sep 29, 2012·Immunological Investigations·Samantha SolitoSusanna Mandruzzato

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.