Abstract
The effects of interferon-gamma (IFN-gamma) on protein synthesis of a pre-B cell leukemia, L1210, have been studied by two-dimensional gel electrophoresis. In total cell extracts, at least ten proteins were induced de novo, or increased in their expression, after an 18-h IFN-gamma treatment, whereas in the nuclear extracts eight proteins were specifically induced. Of these, increased synthesis of a 40-kDa/pI 5.9 cytoplasmic protein was the most prominent and reproducible. Most of these proteins appear to be specific for a defined step of differentiation, since they are not found in other B cell leukemias upon IFN-gamma treatment. Others appear to be tissue specific, since they are not induced in fibroblasts nor in T cells. In addition, synthesis of some of the induced proteins appeared to require rapid transcription of new mRNA, because actinomycin D markedly inhibited their formation when added immediately before IFN-gamma. In keeping with this finding, in vitro translation of mRNA from IFN-gamma-treated L1210 cells into a rabbit reticulocyte lysate system, followed by analysis of the labeled proteins by two-dimensional gel electrophoresis, revealed the appearance of at least seven proteins. Taken as a whole, these results dem...Continue Reading
References
Nov 27, 1979·Biochemistry·J M ChirgwinW J Rutter
Jun 1, 1977·Nucleic Acids Research·Y Y Mory, M L Gefter
Oct 1, 1987·The Journal of Experimental Medicine·G KaplanZ A Cohn
Jun 1, 1988·Journal of Virology·B Y RubinL J Smith
Apr 1, 1988·European Journal of Immunology·S LandolfoG Cavallo
Jun 1, 1986·The Journal of General Virology·F CofanoS Landolfo
Jan 1, 1986·Current Topics in Microbiology and Immunology·M M SimonU Hochgeschwender
Aug 1, 1988·Journal of Interferon Research·M GariglioS Landolfo
Jun 20, 1985·Nature·A D LusterJ V Ravetch
May 1, 1980·Journal of Virology·B Y Rubin, S L Gupta
Apr 1, 1982·European Journal of Immunology·S LandolfoM M Simon
Jan 1, 1982·Annual Review of Biochemistry·P Lengyel
Oct 15, 1983·Virology·K Linnavuori, T Hovi
Jan 1, 1983·Advances in Immunology·R M Friedman, S N Vogel
Jan 1, 1984·Pharmacology & Therapeutics·G C Sen
Jan 1, 1984·Pharmacology & Therapeutics·K C Zoon, H Arnheiter
Oct 27, 1983·The New England Journal of Medicine·C P MauryA M Teppo
Jan 1, 1982·Immunological Reviews·R L Coffman