PMID: 8039012Oct 1, 1993

Characterization of cytoskeletal and neuronal markers in micromass cultures of rat embryonic midbrain cells

Cell Biology and Toxicology
S G WhittakerE M Faustman

Abstract

Micromass cultures of rat embryonic midbrain cells were characterized with regard to the immunolocalization of neuronal and cytoskeletal markers. Cells taken from gestational day-12 embryos and cultured for 5 days in vitro comprise at least two morphologically distinct cells types: fibroblast-like cells and neurons. Antibodies to the following markers yielded preferential staining of neuronal cells: A2B5 (GQ ganglioside), gamma-aminobutyric acid (GABA), microtubule-associated protein 2 (MAP2), MAP5, neuron-specific enolase (NSE), neural cell adhesion molecule (N-CAM), and tau. Antibodies to beta-tubulin, c-neu, MAP1, and neurofilament (NF-H) stained both neuronal and fibroblast-like cells. Antibodies to glial fibrillary acidic protein (GFAP) and vimentin failed to immunoreact with any cells in day-5 CNS cultures. SDS-PAGE and Western analysis were employed to determine the specificity of the antibodies and determine the electrophoretic profiles of the markers. We conclude that the pattern of neuronal differentiation in CNS micromass cultures exhibits certain similarities to that observed in vivo. In addition, certain markers identified in this study may be of potential utility as (1) biomarkers of chemically-induced development...Continue Reading

References

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Citations

Nov 12, 2002·Environmental Health Perspectives·E M FaustmanT J Kavanagh
Apr 1, 2009·International Journal of Molecular Sciences·Iwona Wilk-Zasadna, Maria Minta
Oct 28, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jaspreet S SidhuE M Faustman
May 1, 2001·Current Protocols in Toxicology·R A Ponce

Related Concepts

Metazoa
Microtrabecular Lattice
Embryonic and Fetal Development
Immunocytochemistry
Midbrain Structure
Micromanipulation
Nerve Tissue Proteins
Neurons
Western Blot
Viral Markers

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