Characterization of DegQVh, a serine protease and a protective immunogen from a pathogenic Vibrio harveyi strain.

Applied and Environmental Microbiology
Wei-wei ZhangLi Sun

Abstract

Vibrio harveyi is an important marine pathogen that can infect a number of aquaculture species. V. harveyi degQ (degQ(Vh)), the gene encoding a DegQ homologue, was cloned from T4, a pathogenic V. harveyi strain isolated from diseased fish. DegQ(Vh) was closely related to the HtrA family members identified in other Vibrio species and could complement the temperature-sensitive phenotype of an Escherichia coli strain defective in degP. Expression of degQ(Vh) in T4 was modulated by temperature, possibly through the sigma(E)-like factor. Enzymatic analyses demonstrated that the recombinant DegQ(Vh) protein expressed in and purified from E. coli was an active serine protease whose activity required the integrity of the catalytic site and the PDZ domains. The optimal temperature and pH of the recombinant DegQ(Vh) protein were 50 degrees C and pH 8.0. A vaccination study indicated that the purified recombinant DegQ(Vh) was a protective immunogen that could confer protection upon fish against infection by V. harveyi. In order to improve the efficiency of DegQ(Vh) as a vaccine, a genetic construct in the form of the plasmid pAQ1 was built, in which the DNA encoding the processed DegQ(Vh) protein was fused with the DNA encoding the secret...Continue Reading

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Citations

Jan 12, 2013·Applied Microbiology and Biotechnology·Weiwei ZhangLingxin Chen
Nov 27, 2009·The Journal of General and Applied Microbiology·Kun SunLi Sun
Feb 27, 2014·Veterinary Research·Yong-Hua HuLi Sun
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Emrah AltindisJohn J Mekalanos
Jun 19, 2012·Fish & Shellfish Immunology·Ren-ping JinLi Sun
Oct 1, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Ling Chen, Li Sun
Sep 6, 2011·Fish & Shellfish Immunology·Yong-hua HuLi Sun
Apr 19, 2011·Fish & Shellfish Immunology·Jin-sheng SunLi Sun
Aug 10, 2010·Developmental and Comparative Immunology·Ping-ping XiaoLi Sun
Apr 13, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Wen-jiang ZhengLi Sun
Jan 12, 2010·Fish & Shellfish Immunology·Xu-dong JiaoLi Sun
Sep 29, 2009·Veterinary Microbiology·Kun SunLi Sun

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