Characterization of dendritic cells and follicular dendritic cells in the hepatic lymph nodes and liver of sheep experimentally infected with Fasciola hepatica

Veterinary Research
María Teresa Ruiz-CampilloJ Pérez

Abstract

Fasciola hepatica has been shown to have a high capacity for immunomodulation of the host response, making the development of protective vaccines extremely difficult. One of these immunomodulation mechanisms is the impairment of dendritic cells (DC) maturation and, therefore, suppression of antigenic presentation. The aim of this study was to evaluate the pathological changes as well as the characterization of two antigen presenting cells, DC (CD1b, CD83 and MHC-II positive) and follicular dendritic cells (FDC) (CNA.42, S100 and CD83 positive) by immunohistochemistry in the hepatic lymph nodes (HLN) and livers of sheep during the early stages of infection with F. hepatica [9 and 18 days post-infection (dpi)], compared with an uninfected group (UC) as a control. The results revealed a marked hyperplasia of HLN germinal centres at 9 and, in particular, 18 dpi, with respect to the UC group, with coincidental increased expression of CNA.42 in FDC of lymphoid follicles and CD1b in the DC of paracortical areas at 18 dpi. However, the expression of MHC-II and CD83 decreased at 9 and, particularly, at 18 dpi in HLN compared with that in the UC group. Since both markers are related to active presentation of antigens by DC and FDC, the r...Continue Reading

References

Feb 15, 2000·Parasite Immunology·S M O'NeillJ P Dalton
Mar 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·N SchollerJ A Ledbetter
Mar 20, 2001·Veterinary Research·A ChauvinC Boulard
Apr 11, 2003·Medical Microbiology and Immunology·Matthias WolenskiArne von Bonin
Sep 13, 2005·Journal of Helminthology·S Mas-Coma
Nov 23, 2006·The Journal of Experimental Medicine·Louise A JohnsonDavid G Jackson
Feb 12, 2008·Seminars in Immunology·Christopher D C Allen, Jason G Cyster
Apr 1, 2009·Infection and Immunity·Clare M HamiltonSandra M O'Neill
Apr 16, 2009·Journal of Helminthology·I Fairweather
Apr 22, 2009·The Journal of Experimental Medicine·Arielle Glatman ZaretskyEdward J Pearce
Apr 22, 2009·The Journal of Experimental Medicine·Irah L King, Markus Mohrs
Jul 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kevin P WalshKingston H G Mills
May 8, 2010·Veterinary Immunology and Immunopathology·Cristian FalcónLaura Cervi
Dec 12, 2012·The Korean Journal of Parasitology·Joon-Yong ChungYoon Kong
Sep 10, 2014·International Journal for Parasitology·Hayley ToetTerry W Spithill
Feb 7, 2015·Veterinary Parasitology·Verónica Molina-HernándezKrystyna Cwiklinski
Jan 1, 2016·PLoS Neglected Tropical Diseases·Ernesto RodríguezTeresa Freire
Mar 18, 2016·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lena KrzyzakLars Nitschke

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad Prism
GraphPad
ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.