Characterization of Diaphanous-related formin FMNL2 in human tissues.

BMC Cell Biology
Maria GardbergOlli Carpén

Abstract

Diaphanous-related formins govern actin-based processes involved in many cellular functions, such as cell movement and invasion. Possible connections to developmental processes and cellular changes associated with malignant phenotype make them interesting study targets. In spite of this, very little is known of the tissue distribution and cellular location of any mammalian formin. Here we have carried out a comprehensive analysis of the formin family member formin -like 2 (FMNL2) in human tissues. An FMNL2 antibody was raised and characterized. The affinity-purified FMNL2 antibody was validated by Western blotting, Northern blotting, a peptide competition assay and siRNA experiments. Bioinformatics-based mRNA profiling indicated that FMNL2 is widely expressed in human tissues. The highest mRNA levels were seen in central and peripheral nervous systems. Immunohistochemical analysis of 26 different human tissues showed that FMNL2 is widely expressed, in agreement with the mRNA profile. The widest expression was detected in the central nervous system, since both neurons and glial cells expressed FMNL2. Strong expression was also seen in many epithelia. However, the expression in different cell types was not ubiquitous. Many mesenc...Continue Reading

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Citations

Jun 29, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Timo T MyöhänenOlli Carpen
Aug 9, 2016·The Journal of Pathology. Clinical Research·Maria GardbergOlli Carpén
Jun 11, 2014·Small GTPases·Sonja Kühn, Matthias Geyer
Aug 15, 2015·Gene Expression Patterns : GEP·Priyanka Dutta, Sankar Maiti
May 13, 2015·The Journal of Cell Biology·Katharina GrikscheitRobert Grosse
Oct 19, 2012·Cell Adhesion & Migration·Tobias ZechLaura M Machesky
Jun 28, 2016·PloS One·Vinicius Henrique da SilvaLuiz Lehmann Coutinho
Apr 5, 2014·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Maria GardbergOlli Carpén
Nov 3, 2017·Nature Communications·Stéphanie Miserey-LenkeiBruno Goud
Apr 16, 2020·The Journal of Cell Biology·Karin PfistererMaddy Parsons
Aug 29, 2020·Small GTPases·Ananthalakshmy Sundararaman, Harry Mellor
Mar 3, 2012·G3 : Genes - Genomes - Genetics·Yu-Ching ChengBraxton D Mitchell
Jul 14, 2012·Bioscience, Biotechnology, and Biochemistry·Koko MoriyaToshihiko Utsumi

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Methods Mentioned

BETA
GTPases
electrophoresis
transfection
GTPase
confocal microscopy

Software Mentioned

Image J

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