Characterization of different alginate lyases for dissolving Pseudomonas aeruginosa biofilms.

Scientific Reports
Núria Blanco-CabraMaria LLuch-Senar

Abstract

Aggregates of Pseudomonas aeruginosa form a protective barrier against antibiotics and the immune system. These barriers, known as biofilms, are associated with several infectious diseases. One of the main components of these biofilms is alginate, a homo- and hetero-polysaccharide that consists of β-D-mannuronate (M) and α-L-guluronate (G) units. Alginate lyases degrade this sugar and have been proposed as biotherapeutic agents to dissolve P. aeruginosa biofilms. However, there are contradictory reports in the literature regarding the efficacy of alginate lyases against biofilms and their synergistic effect with antibiotics. We found that most positive reports used a commercial crude extract from Flavobacterium multivorum as the alginate lyase source. By using anion exchange chromatography coupled to nano LC MS/MS, we identified two distinct enzymes in this extract, one has both polyM and polyG (polyM/G) degradation activities and it is similar in sequence to a broad-spectrum alginate lyase from Flavobacterium sp. S20 (Alg2A). The other enzyme has only polyG activity and it is similar in sequence to AlyA1 from Zobellia galactanivorans. By characterizing both of these enzymes together with three recombinant alginate lyases (a po...Continue Reading

References

Jan 1, 1977·Journal of General Microbiology·I W DavidsonI W Sutherland
Apr 1, 1992·The International Journal of Biochemistry·P Gacesa
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1986·European Journal of Clinical Microbiology·F FollathH Reber
Oct 15, 1986·Carbohydrate Research·G Skjåk-BraekB Larsen
Aug 22, 2001·Journal of Bacteriology·M HentzerM R Parsek
Aug 28, 2002·International Journal of Medical Microbiology : IJMM·Philip S Stewart
Apr 20, 2004·Journal of Bacteriology·Osamu MiyakeKousaku Murata
Aug 6, 2005·Journal of Molecular Biology·Masayuki YamasakiKousaku Murata
Nov 23, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jeff G LeidAlyssa K Jeffers
Mar 8, 2006·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Mustafa A AlkawashNeal L Schiller
May 18, 2006·The International Journal of Artificial Organs·U ObstH Volkmann
Jun 1, 2007·Clinical Pharmacology and Therapeutics·J L del Pozo, R Patel
Oct 8, 2008·Nucleic Acids Research·Brandi L CantarelBernard Henrissat
Apr 28, 2009·Respiratory Care·Jane C Davies, Diana Bilton
Oct 22, 2009·Applied Microbiology and Biotechnology·Ryoji MatsushimaMasataka Tsuda
Dec 3, 2009·Proteomics·Joerg SeidlerWolf D Lehmann
Feb 13, 2010·International Journal of Antimicrobial Agents·Niels HøibyOana Ciofu
Oct 17, 2012·Antimicrobial Agents and Chemotherapy·John W Lamppa, Karl E Griswold
Apr 29, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Aida BaeloEduard Torrents

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Methods Mentioned

BETA
acetylation
gel filtration
electrophoresis
confocal microscopy

Software Mentioned

ImageJ
Basic Local Alignment search Tool ( BLAST )
PEAKS
GraphPad Prism
BLAST
Image J
COMSTAT
GraphPad
ClustalW

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