Nov 1, 1975

Characterization of different deoxyribonucleases in human lymphocytes

Zeitschrift Für Naturforschung. Section C: Biosciences
E J ZöllnerR K Zahn


Four groups of deoxyribonuclease activities from human lymphocytes have been characterized by deoxyribonuclease assay in DNA-containing polyacrylamide gels following their separation by disc-electrophoresis. All activities hydrolyse DNA endonucleolytically. One neutral deoxyribonuclease found in the cytoplasmic fraction prefers native or UV-irradiated DNA over denatured DNA as substrate and is a 5'-monoester former. Two groups of acid deoxyribonuclease activities are detectable in the nuclear fraction. Both are 3'-monoester formers. One is as well active with denatured DNA as with native DNA, the other one shows the same activity with native and UV-irradiated DNA but lower activity with denatured DNA. An alkaline deoxyribonuclease activity, also localized in the nucleus, is a 5'-monoester former, and prefers denatured or UV-irradiated DNA as substrate.

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Mentioned in this Paper

Polyacrylamide Gels
RNA Denaturation
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Lymphoid Cells
Effects of Radiation
Cell Nucleus
Alkaline DNase
Electrophoresis, Disc

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