Characterization of different plasmid-borne dihydropteroate synthases mediating bacterial resistance to sulfonamides.

Journal of Bacteriology
G Swedberg, O Sköld

Abstract

Plasmid-borne resistance to sulfonamides was studied in both newly isolated and earlier characterized R plasmids. Two different classes of drug-resistant dihydropteroate synthases were found to be responsible for most cases of plasmid-mediated sulfonamide resistance. The plasmid-coded enzymes could be completely separated from their chromosomal counterpart and also showed differences in heat stability and molecular size. The resistant and chromosomal enzymes could bind the normal substrate, p-aminobenzoic acid, with equal efficiency. In contrast, sulfonamide binding was about 10,000 times lower with the plasmid-coded enzymes than with the chromosomal enzyme. Another substrate analog, p-aminosalicylic acid, on the other hand, inhibited chromosomal and plasmid-mediated enzymes to a similar extent. Evidence was also found for the existence of a plasmid-borne resistance mechanism independent of drug-insensitive enzymes.

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Citations

May 4, 2010·Applied Microbiology and Biotechnology·Saeed M Hashimi, Robert G Birch
Oct 12, 2019·International Journal of Molecular Sciences·Daniel Fernández-VillaLuis Rojo
Jan 1, 1996·Microbiology and Immunology·E H Kim, T Aoki
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Jul 16, 2019·MedChemComm·Shannon Lynn Kordus, Anthony David Baughn
Dec 1, 1989·Antimicrobial Agents and Chemotherapy·B P Nichols, G G Guay
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Mar 1, 1987·Microbiological Reviews·B R Lyon, R Skurray
Jan 13, 2018·Microbiology Spectrum·Engeline van DuijkerenStefan Schwarz

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