Characterization of differential pore-forming activities of ESAT-6 proteins from M. tuberculosis and M. smegmatis

FEBS Letters
Xiuli PengJianjun Sun

Abstract

Mycobacterium tuberculosis ESAT-6 (MtbESAT-6) plays essential roles in pathogenesis. MtbESAT-6 exhibits a unique pore-forming activity (PFA) that is not found in its ortholog from non-pathogenic Mycobacterium smegmatis (MsESAT-6). Here we characterized the differential PFAs and found that exchange of I25-H26/T25-A26 between two proteins reciprocally affected their PFAs. MtbESAT-6(IH/TA) had ~40% reduction, while MsESAT-6(TA/IH) fully acquired its activity similar to MtbESAT-6. Mutations of A17E, K38T, N67L or R74Q on MtbESAT-6(IH/TA) further reduced the activity, with MtbESAT-6(IH/TA-17) being the lowest. This study suggests I25-H26 as the pH-sensor essential for MsESAT-6 to fully acquire the activity, while multiple residues contributed to MtbESAT-6 PFA. This article is protected by copyright. All rights reserved.

References

Oct 29, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·B J WardsD M Collins
Jan 1, 2003·The Journal of Infectious Diseases·Kaeryn N LewisDavid R Sherman
Apr 15, 2003·Nature Medicine·Alexander S PymStewart T Cole
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tsungda HsuWilliam R Jacobs
Mar 24, 2004·Current Opinion in Microbiology·Ian Orme
Aug 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jessica L FlintKeith M Derbyshire
Jul 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·S M FortuneE J Rubin
Oct 6, 2005·The Journal of Experimental Medicine·Peter B KangLarry S Schlesinger
Feb 25, 2006·Cell·Javier Pizarro-Cerdá, Pascale Cossart
Nov 17, 2006·The Journal of Biological Chemistry·Jianjun SunR John Collier
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sarah A StanleyJeffery S Cox
Apr 17, 2007·Microbiological Research·Janka TeutschbeinThomas Munder
Jun 17, 2008·Molecular Microbiology·Abbie CorosKeith M Derbyshire
May 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anthony A RyanJames A Triccas
Jul 16, 2010·Molecular Systems Biology·Christian PoulsenYoung-Hwa Song
Apr 25, 2012·Cellular Microbiology·Diane HoubenPeter J Peters
Feb 22, 2013·The New England Journal of Medicine·Alimuddin ZumlaC Fordham von Reyn
Mar 12, 2013·Bulletin of the World Health Organization
Apr 5, 2013·Bulletin of the World Health Organization·Haileyesus GetahunMario Raviglione
Jul 16, 2013·Cellular Microbiology·J I AguiloCarlos Martin
Aug 21, 2013·Primary Care·Wanda Cruz-Knight, Lyla Blake-Gumbs
Dec 20, 2014·Cell·C J CambierLalita Ramakrishnan
Jun 7, 2015·Cell Host & Microbe·Angela C CollinsMichael U Shiloh

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Citations

Jun 2, 2016·Cellular Microbiology·David G Russell
Oct 1, 2016·Current Infectious Disease Reports·Taojun HeYi-Wei Tang
Jan 26, 2017·Proceedings of the National Academy of Sciences of the United States of America·William H ConradLalita Ramakrishnan
May 4, 2017·Journal of Bacteriology·Rachel E Bosserman, Patricia A Champion
Mar 15, 2020·The Journal of Biological Chemistry·Javier AguileraJianjun Sun
Dec 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Julia LienardFredric Carlsson
Dec 8, 2016·British Journal of Pharmacology·Nor Fadhilah KamaruzzamanLiam Good
Sep 15, 2020·Frontiers in Cellular and Infection Microbiology·Jacques AugenstreichCatherine Astarie-Dequeker
Sep 4, 2020·Microbiology and Molecular Biology Reviews : MMBR·Alexandra E ChirakosPatricia A Champion
Sep 10, 2020·Infection and Immunity·Alexandra E ChirakosPatricia A Champion
Jan 17, 2021·Nature Communications·Uday TakMichael Niederweis
Feb 1, 2021·The Journal of Biological Chemistry·Javier AguileraJianjun Sun
Apr 6, 2021·Critical Reviews in Microbiology·Avijit GoswamiRachit Agarwal

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