PMID: 2121705Sep 1, 1990Paper

Characterization of differentiated Syrian golden hamster pancreatic duct cells maintained in extended monolayer culture

In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
S HubchakD G Scarpelli

Abstract

Epithelial cells isolated from fragments of hamster pancreas interlobular ducts were freed of fibroblast contamination by plating them on air-dried collagen, maintaining them in serum-free Dulbecco's modified Eagle's (DME):F12 medium supplemented with growth factors, and selecting fibroblast-free aggregates of duct cells with cloning cylinders. Duct epithelial cells plated on rat type I collagen gel and maintained in DME:F12 supplemented with Nu Serum IV, bovine pituitary extract, epidermal growth factor, 3,3',5-triiodothyronine, dexamethasone, and insulin, transferrin, selenium, and linoleic acid conjugated to bovine serum albumin (ITS+), showed optimal growth as monolayers with a doubling time of about 20 h and were propagated for as long as 26 wk. Early passage cells consisted of cuboidal cells with microvilli on their apical surface, complex basolateral membranes, numerous elongated mitochondria, and both free and membrane-bound ribosomes. Cells grown as monolayers for 3 mo. were more flattened and contained fewer apical microvilli, mitochondria, and profiles of rough surfaced endoplasmic reticulum; in addition, there were numerous autophagic vacuoles. Functional characteristics of differentiated pancreatic duct cells which...Continue Reading

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Citations

Apr 29, 1998·In Vitro Cellular & Developmental Biology. Animal·D OdaS P Lee
Dec 20, 1996·Cancer Letters·C A CatesD N Crowell
Feb 1, 1995·In Vitro Cellular & Developmental Biology. Animal·S YuanL Rosenberg
Jun 9, 1999·International Journal of Cancer. Journal International Du Cancer·A ChlenskiD G Scarpelli
Feb 21, 2002·Pancreas·Alexis B UlrichParviz M Pour

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