Characterization of dimethylargininase from bovine brain: evidence for a zinc binding site

Biochemistry
R BogumilM Vasák

Abstract

Dimethylargininase (EC 3.5.3.18) is involved in the regulation of the levels of the natural occurring free arginine derivatives L-Nomega,Nomega-dimethylarginine and L-Nomega-methylarginine, which are reversible inhibitors of nitric oxide synthase. A dimethylargininase has been isolated from bovine brain tissue and was characterized by using immunological, kinetic, and spectroscopic techniques. Western blot analysis using polyclonal antibodies revealed that the enzyme is widely distributed in bovine with the highest relative concentrations found in brain and kidney tissue. A similar tissue distribution has also been reported for the other so far isolated dimethylargininase from rat kidney [Ogawa, T., Kimoto, M., and Sasaoka, K. (1989) J. Biol. Chem. 264, 10205-10209]. The bovine enzyme is a monomeric, globular protein (molecular mass approximately 31.2 kDa) containing one tightly bound Zn2+ ion, which can be removed by dialysis against 1,10-phenanthroline. The determination of kinetic constants for both the native (holo-protein) and the zinc-depleted (apo-protein) enzyme at 37 degrees ¿C established that the dimethylargininase is not a zinc hydrolase. The specific activity was 0.66 unit/mg for the holo-protein and 0.19 unit/mg f...Continue Reading

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Citations

Jun 16, 2009·Neurotoxicity Research·Gjumrakch AlievValentin Bragin
Jul 5, 2005·Analytical Biochemistry·Everett M Stone, Walter Fast
Jun 11, 2002·Annual Review of Nutrition·Guoyao Wu, Cynthia J Meininger
Apr 1, 2009·Expert Opinion on Drug Metabolism & Toxicology·Carol Wadham, Arduino A Mangoni
Mar 6, 2007·Journal of the Neurological Sciences·Xiongwei ZhuGeorge Perry
Nov 6, 2012·Chembiochem : a European Journal of Chemical Biology·Karin S AltmannBernd Clement
Apr 24, 2004·Arteriosclerosis, Thrombosis, and Vascular Biology·Patrick Vallance, James Leiper
Oct 16, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Fredrik PalmChristopher S Wilcox
Nov 21, 2002·The Journal of Biological Chemistry·Markus KnippMilan Vasák
Dec 5, 1998·Free Radical Biology & Medicine·M A SmithG Perry

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