PMID: 6407531Feb 7, 1983Paper

Characterization of discoidal complexes of phosphatidylcholine, apolipoprotein A-I and cholesterol by gradient gel electrophoresis

Biochimica Et Biophysica Acta
A V NicholsT M Forte

Abstract

Complexes of egg yolk phosphatidylcholine and apolipoprotein A-I were prepared by a detergent (sodium cholate)-dialysis method and characterized by gradient gel electrophoresis, gel filtration, electron microscopy and chemical analysis. Multicomponent electrophoretic patterns were obtained indicating formation of at least eight classes of discoidal complexes. The relative contribution of the different classes to the electrophoretic pattern was a function of the molar ratio of phosphatidylcholine:apolipoprotein A-I in the interaction mixture. Molar ratios of phosphatidylcholine:apolipoprotein A-I in isolated complexes were strongly and positively correlated with disc diameter obtained by electron microscopy. Incorporation of unesterified cholesterol into phosphatidylcholine/apolipoprotein A-I interaction mixtures also resulted in formation of unique complexes but with considerably different particle size distributions relative to those observed in the absence of cholesterol. One common consequence of cholesterol incorporation into interaction mixtures of 87.5:1 and 150:1 molar ratio of phosphatidylcholine:apolipoprotein A-I was the disappearance of a major complex class with diameter of 10.8 nm and the appearance of a major comp...Continue Reading

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Citations

Oct 1, 1992·Molecular and Chemical Neuropathology·J R MoskalH Lander
Nov 5, 2010·The Journal of Biological Chemistry·Jens O LagerstedtMichael N Oda
Nov 20, 2009·BMC Clinical Pharmacology·Leonid GaidukovEtgar Levy-Nissenbaum
Dec 11, 2008·Journal of Lipid Research·Malgorzata WróblewskaTadeusz Badzio
Mar 6, 2008·Expert Opinion on Drug Delivery·Robert O Ryan
Jan 23, 2016·Frontiers in Pharmacology·Valentin Gogonea
Jan 30, 2013·Biochimica Et Biophysica Acta·Masakazu MiyazakiMinoru Nakano
Jan 1, 1990·Proteins·J P SegrestG M Anantharamaiah
May 20, 2003·Nature Structural Biology·Michael N OdaJohn C Voss
Jun 9, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M R McCallB Frei
Oct 7, 2018·Protein Science : a Publication of the Protein Society·Rita Del Giudice, Jens O Lagerstedt
Mar 3, 1995·The Journal of Biological Chemistry·Q H MengY L Marcel
Dec 1, 1993·Scandinavian Journal of Clinical and Laboratory Investigation·J WestmanE Wülfert
Mar 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T M Forte, L A Carlson
May 16, 2006·The Journal of Biological Chemistry·Dale D O MartinMichael N Oda
Nov 26, 2019·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Oktawia NilssonJens O Lagerstedt
May 17, 1995·Biochimica Et Biophysica Acta·C G Brouillette, G M Anantharamaiah
Aug 4, 1994·Biochimica Et Biophysica Acta·E L GongA V Nichols

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