Characterization of distinct forms of cytochromes P-450, epoxide metabolizing enzymes and UDP-glucuronosyltransferases in rat skin

Biochemical Pharmacology
M A PhamBruce D Hammock

Abstract

Study of drug metabolizing enzyme activity was undertaken in skin microsomal and cytosolic fractions of male and female rats. The presence of several isoforms was revealed from their activities towards selected substrates and from their cross immunoreactivity using antibodies raised against purified hepatic or renal cytochromes P-450, epoxide hydrolase and UDP-glucuronosyltransferases. Cytochrome P-450 content was precisely quantified by second derivative spectrophotometry, 23.1 and 16.5 pmol/mg protein in males and females, respectively. The monooxygenase activity associated to cytochromes P-450IIB1 and P-450IA1 was determined through O-dealkylation of ethoxy-; pentoxy- and benzoxyresorufin. The activity ranged between 4 and 2 nmol/min/mg protein for male and female rats, respectively. These results and Western blot analysis indicated that rat skin microsomes contain both monooxygenase systems associated with cytochromes P-450IIB1 and P-450IA1. By contrast lauric acid hydroxylation, supported by cytochrome P-450IVA1, was not detectable. Activities of epoxide metabolizing enzymes (microsomal and cytosolic epoxide hydrolases; glutathione S-transferase) were also characterized in skin. Microsomes catalysed the hydratation of benz...Continue Reading

References

Sep 1, 1979·Analytical Biochemistry·P M DansetteD M Jerina
Jul 1, 1976·Analytical Biochemistry·G S RaoH Breuer
Jan 1, 1987·Molecular Aspects of Medicine·B BurchellR Hume
Jul 1, 1987·Journal of Neuroscience Methods·J F Ghersi-EgeaG Siest
Jun 1, 1987·Analytical Biochemistry·A ZhiriG Siest
Jun 11, 1986·Brain Research·B WaltherG Siest
May 31, 1985·Biochemical and Biophysical Research Communications·P AsokanH Mukhtar
Dec 1, 1985·The British Journal of Dermatology·M J FinnenS Shuster
Jun 15, 1981·Biochemical Pharmacology·I Okulicz-KozarynV Loppinet

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Citations

Sep 1, 1993·The International Journal of Biochemistry·B S WinderB D Hammock
Feb 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·W W Lovell, D J Sanders
Jan 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W W Lovell
Feb 1, 1995·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B HirelA Guillouzo
Nov 1, 2003·Archives of Biochemistry and Biophysics·Scott A MonkRobert H Rice
Mar 17, 2004·Toxicology and Applied Pharmacology·Liping DuDiane S Keeney
Mar 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P Nasseri-SinaJ Caldwell
Mar 21, 2000·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·T MossF M Williams
Jan 1, 1995·The British Journal of Dermatology·F VecchiniB Shroot
Oct 1, 1991·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·B M HenrikusH G Kampffmeyer
Apr 27, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Carine JacquesDaniel Zalko
Mar 8, 2005·Progress in Lipid Research·John W NewmanBruce D Hammock
Aug 12, 2004·The Journal of Investigative Dermatology·Nihal Ahmad, Hasan Mukhtar
Jan 1, 1990·Drug Metabolism Reviews·J Kao, M P Carver
Dec 7, 2007·Drug Metabolism Reviews·Franz OeschRobert Landsiedel
Feb 22, 2014·Drug Metabolism and Drug Interactions·Hanan Osman-PonchetBernard Ruty
Mar 20, 1992·Journal of Chromatography·V Lang
Jul 16, 1999·Biochemical and Biophysical Research Communications·C CheungS A Hotchkiss
Sep 11, 2002·Biochemical and Biophysical Research Communications·Zheying ZhuRobert J Edwards
Jun 1, 1990·The Journal of Investigative Dermatology·M A PhamB Shroot
Mar 1, 1992·The Journal of Investigative Dermatology·J I AdemolaH I Maibach
Jul 1, 1993·The Journal of Investigative Dermatology·A IannoneA Tomasi
Dec 15, 2007·Biochimica Et Biophysica Acta·Hiromasa TanakaBruce D Hammock

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