Characterization of DLK1(PREF1)+/CD34+ cells in vascular stroma of human white adipose tissue

Stem Cell Research
Marit E ZwierzinaWerner Zwerschke

Abstract

Sorting of native (unpermeabilized) SVF-cells from human subcutaneous (s)WAT for cell surface staining (cs) of DLK1 and CD34 identified three main populations: ~10% stained cs-DLK1+/cs-CD34-, ~20% cs-DLK1+/cs-CD34+dim and ~45% cs-DLK1-/cs-CD34+. FACS analysis after permeabilization showed that all these cells stained positive for intracellular DLK1, while CD34 was undetectable in cs-DLK1+/cs-CD34- cells. Permeabilized cs-DLK1-/cs-CD34+ cells were positive for the pericyte marker α-SMA and the mesenchymal markers CD90 and CD105, albeit CD105 staining was dim (cs-DLK1-/cs-CD34+/CD90+/CD105+dim/α-SMA+/CD45-/CD31-). Only these cells showed proliferative and adipogenic capacity. Cs-DLK1+/cs-CD34- and cs-DLK1+/cs-CD34+dim cells were also α-SMA+ but expressed CD31, had a mixed hematopoietic and mesenchymal phenotype, and could neither proliferate nor differentiate into adipocytes. Histological analysis of sWAT detected DLK1+/CD34+ and DLK1+/CD90+ cells mainly in the outer ring of vessel-associated stroma and at capillaries. DLK1+/α-SMA+ cells were localized in the CD34- perivascular ring and in adventitial vascular stroma. All these DLK1+ cells possess a spindle-shaped morphology with extremely long processes. DLK1+/CD34+ cells were a...Continue Reading

References

Jul 18, 2002·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·D B HausmanR J Martin
Feb 18, 2003·The Journal of Clinical Investigation·Kichoon LeeHei Sook Sul
Dec 5, 2006·The Journal of Clinical Investigation·Joseph T CrossnoDwight J Klemm
Jul 24, 2007·Journal of Dermatological Science·Naoki YamamotoKayoko Matsunaga
Oct 25, 2007·Cell·Stephane GestaC Ronald Kahn
Apr 10, 2008·Cell Stem Cell·Paolo BiancoPaul J Simmons
Jul 4, 2008·Stem Cells and Development·Guiting LinChing-Shwun Lin
Sep 20, 2008·Science·Wei TangJonathan M Graff
Oct 7, 2008·Cell·Matthew S RodehefferJeffrey M Friedman
Mar 4, 2009·Cell Metabolism·Yuhui Wang, Hei Sook Sul
Jun 23, 2009·Molecular Endocrinology·Hei Sook Sul
Jul 7, 2009·Experimental Hematology·Yasuhiko SeraMakio Ogawa
Nov 26, 2010·Nature Medicine·Damian MediciBjorn R Olsen
Jan 27, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M MaumusP Bourin
Mar 17, 2011·PloS One·Alessandra RigamontiChad A Cowan
May 6, 2011·Stem Cells·Susan M MajkaDwight J Klemm
May 17, 2011·Plastic and Reconstructive Surgery·Han LiJ Peter Rubin
Jun 18, 2011·Biology of the Cell·Xiaoxiao CaiBrian E Grottkau
Jul 13, 2011·Bone·Basem M Abdallah, Moustapha Kassem
Aug 25, 2011·Stem Cells and Development·Mirko CorselliBruno Péault
Nov 10, 2011·Cardiovascular Research·Patricia RodríguezSantiago Lamas
Nov 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Ludovic ZimmerlinAlbert D Donnenberg
Feb 26, 2013·Nature Cell Biology·Ryan Berry, Matthew S Rodeheffer
May 10, 2013·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Maria C MitterbergerWerner Zwerschke
Aug 16, 2013·Development·Ditte Caroline AndersenCharlotte Harken Jensen
Sep 21, 2013·Development·Daniel C BerryJonathan M Graff
Sep 21, 2013·Adipocyte·Gunnhildur Asta TraustadottirDitte C Andersen
Feb 6, 2014·Stem Cells·Laura E SidneyAndrew Hopkinson
Jan 1, 2013·Journal of Genomics·Gary J Hausman, Michael V Dodson

❮ Previous
Next ❯

Citations

Dec 3, 2016·Experimental Biology and Medicine·Ignazio BarbagalloLuca Vanella
Apr 1, 2018·Experimental Dermatology·Carina NicuRalf Paus
Mar 1, 2019·Cellular & Molecular Biology Letters·Claudia KrautgasserWerner Zwerschke
Jun 13, 2020·International Journal of Molecular Sciences·Carina da SilvaMichael S Pepper
Oct 19, 2019·Biological Reviews of the Cambridge Philosophical Society·Xin Su, Daoquan Peng
Aug 17, 2019·Pharmacology & Therapeutics·Asim EjazPeter J Rubin
Jan 26, 2021·The Journal of Investigative Dermatology·Carina NicuRalf Paus
May 27, 2017·Stem Cell Research·Susanne HörlWerner Zwerschke

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.