PMID: 46284Feb 1, 1975

Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells

Journal of Virology
D L RobertsonR E Thach


The RNA-dependent DNA polymerase present in intracisternal A-type particles from mouse myeloma tumor cells has been studied. This polymerase can use either endogenous A particle RNA or an exogenous synthetic polynucleotide [poly (rA)] as a template. The DNA reaction product is small (4S-10S) and over 90% of it hybridizes to A particle RNA, whereas up to 50% of it hybridizes to murine sarcoma-leukemia virus RNAs. The RNA isolated from purified A particles is generally of low molecular weight (5S-15S) but contains small amount of 70S and 35S components. These results suggest that A-type particles may be related to C-type oncornaviruses.


Jan 1, 1980·European Journal of Biochemistry·A B ChepelinskyN A Wivel
Mar 1, 1977·European Journal of Biochemistry·J SchmidtB J Weimann
Jan 1, 1978·The Journal of Comparative Neurology·S C Landis, R J Mullen

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Centrifugation, Density Gradient
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