Characterization of DNASE I hypersensitive sites in the 120kb 5' to the CFTR gene

Biochemical and Biophysical Research Communications
A N SmithAnn Harris

Abstract

The chromatin structure of 120kb of genomic DNA 5' to the CFTR gene has been analysed in a number of CFTR expressing and non-expressing cell types, including primary genital duct epithelial cells. Novel DNAse I hypersensitive sites have been observed at -79.5kb and -20.5kb 5' to the ATG translation start codon of the CFTR coding sequence. Neither of these sites appears to show strong correlation with CFTR expression in the cell types investigated, hence they are unlikely to reflect the sites of binding of the major CFTR tissue specific regulator(s). However, they may still play an important part in the complex series of events involved in the regulation of CFTR transcription.

Citations

Nov 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Christopher J OttAnn Harris
Jul 16, 1997·The EMBO Journal·A L MansonC Huxley
Sep 29, 2011·Nucleic Acids Research·Christopher J OttAnn Harris
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