PMID: 2121743Nov 1, 1990Paper

Characterization of dominant and recessive assembly-defective mutations in mouse neurofilament NF-M

The Journal of Cell Biology
P C Wong, D W Cleveland

Abstract

We have generated a set of amino- and carboxy-terminal deletions of the neurofilament NF-M gene and determined the molecular consequences of forced expression of these mutant constructs in mouse fibroblasts. To follow the expression of mutant NF-M subunits in transfected cells, a 12 amino acid epitope (from the human c-myc protein) was expressed at the carboxy terminus of each mutant. We show that NF-M molecules missing up to 90 or 70% of the nonhelical carboxy-terminal tail or amino-terminal head domains, respectively, incorporate readily into an intermediate filament network comprised either of vimentin or NF-L, whereas deletions into either the amino- or carboxy-terminal alpha-helical rod region generate assembly-incompetent polypeptides. Carboxy-terminal deletions into the rod domain invariably yield dominant mutants which rapidly disrupt the array of filaments comprised of NF-L or vimentin. Accumulation of these mutant NF-M subunits disrupts vimentin filament arrays even when present at approximately 1% the level of the wild-type subunits. In contrast, the amino-terminal deletions into the rod produce pseudo-recessive mutants that perturb the wild-type NF-L or vimentin arrays only modestly. The inability of such amino-term...Continue Reading

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Citations

Nov 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R A Quinlan, M Stewart
Sep 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R L Shoeman, P Traub
Sep 1, 1991·Journal of Neuroscience Research·L M ParysekC A Ley
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Feb 14, 2002·Chemical Reviews·Natasha E Zachara, Gerald W Hart
Apr 24, 1999·The European Journal of Neuroscience·M L FeltriL Wrabetz
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Jul 1, 1992·The Journal of Cell Biology·K L VikstromG G Borisy
Feb 1, 1993·The Journal of Cell Biology·D A Compton, D W Cleveland
Apr 1, 1993·The Journal of Cell Biology·S OkabeN Hirokawa
Sep 1, 1993·The Journal of Cell Biology·M K LeeD W Cleveland
May 1, 1994·The Journal of Cell Biology·E Fuchs
Oct 1, 1994·The Journal of Cell Biology·S TakedaN Hirokawa
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Jun 1, 1996·The Journal of Cell Biology·Z XuD W Cleveland
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