Characterization of drug resistant Salmonella enterica serotype Typhimurium by antibiograms, plasmids, integrons, resistance genes and PFGE

Journal of Microbiology and Biotechnology
Douadi BenacerSavithri Devi Puthucheary

Abstract

Forty-seven Salmonella Typhimurium (33 zoonotic, 14 clinical) strains were tested for antimicrobial resistance using the standard disk diffusion method. Presence of relevant resistance genes and class 1 integrons were investigated by using PCR. Pulsed-field gel electrophoresis (PFGE) and plasmid profiling were carried out to determine the genomic diversity of Salmonella Typhimurium. Approximately 57.4% of S. Typhimurium were multidrug resistant (MDR) and showed high resistance rates to tetracycline (70.2%), sulphonamides (57.4%), streptomycin (53.1%), ampicillin (29.7%), nalidixic acid (27.6%), kanamycin (23.4%), chloramphenicol (21.2%) and trimethoprim (19.1%). Resistance towards cephalosporins was noted for cephalothin (27.6%), cephradine (21.2%), amoxicillin clavulanic acid (17.0%) and cephalexin (17.0%). Resistance genes, blaTEM, strA, aadA, sul1, sul2, tet(A), tet(B) and tet(C) were detected among the drug resistant strains. Thirty-three strains (70.2%) carried class 1 integrons, which were grouped in 9 different profiles. DNA sequencing identified sat, aadA, pse-1 and dfrA genes in variable regions on class 1 integrons. Thirty-five strains (74.4%) were subtyped to 22 different plasmid profiles, each with 1 - 6 plasmids (2...Continue Reading

Citations

Oct 17, 2012·Foodborne Pathogens and Disease·Truong Ha ThaiRyoji Yamaguchi
Jan 21, 2012·BMC Research Notes·Pankaj BaralBasudha Shrestha
Aug 9, 2013·International Journal of Environmental Research and Public Health·Antonio CamardaCarlo Pazzani
Oct 22, 2013·Diagnostic Microbiology and Infectious Disease·Soo Tein Ngoi, Kwai Lin Thong
Dec 20, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Kwai Lin ThongCindy Shuan Ju Teh
Jun 25, 2014·Current Opinion in Microbiology·Mary D Barton
Aug 26, 2014·Jundishapur Journal of Microbiology·Fariba AsgharpourZahra Moulana
Aug 28, 2019·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Belgin SirikenIrfan Erol
Sep 19, 2017·Journal of Food Protection·Ting-Ting CaoXiao-Ping Liao
Feb 15, 2017·Tropical Medicine and Health·Linda Aurelia AndohAnders Dalsgaard
Nov 2, 2019·PloS One·Sushim K GuptaMichael McClelland
Dec 23, 2020·Animals : an Open Access Journal From MDPI·Hai Nguyen ThiFabrizio Bertelloni
Mar 30, 2020·Microbiology Spectrum·Wondwossen A GebreyesSiddhartha Thakur

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