PMID: 7541133Apr 1, 1995Paper

Characterization of drug transport through tight-junctional pathway in Caco-2 monolayer: comparison with isolated rat jejunum and colon

Pharmaceutical Research
Y TanakaS Yamashita

Abstract

Drug transport through the tight-junctional pathway in Caco-2 monolayer was studied by examining the relationship between its permeability to hydrophilic drugs and membrane conductance. Compared with the rat isolated jejunum or colon, Caco-2 monolayer displayed high electrical resistance and low conductance, as well as low permeability to sulfanilic acid and FITC-dextran (M.W. 4000). However, there was a linear relationship between the drug permeability and partial Cl- ion conductance for Caco-2 monolayer, rat jejunum and colon. Hence, the permeability to those drugs per unit of Cl- conductance is similar in the three membranes, suggesting that the size (radius) of the tight-junctional pathway in the three membranes is similar. In addition, when the electrical resistance of Caco-2 monolayer was reduced to the same level as that of the jejunum or colon by pretreatment with disodium ethylenediaminetetraacetate, its permeability to FITC-dextran became significantly higher than that of other membranes. Accordingly, the high resistance and the low permeability of Caco-2 monolayer compared with rat intestinal membrane may be due to structural differences between the membranes, rather than a difference in the tightness of the junction.

Citations

Feb 19, 2002·The Journal of Pharmacy and Pharmacology·Toshikiro KimuraKazutaka Higaki
Apr 2, 2005·Drug Delivery·Zelihagül DegimUfuk Abbasoglu
Aug 4, 2009·European Journal of Drug Metabolism and Pharmacokinetics·Da-Hua FuZhi-Min Han
Apr 2, 2005·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Nobuhiro Nishimura
Sep 30, 2006·Acta Pharmacologica Sinica·Er-li MaMing-xing Duan
Dec 22, 2007·Drug Development and Industrial Pharmacy·Herbert HoyerAndreas Bernkop-Schnürch
Nov 19, 2003·AAPS PharmSci·Vanessa M D'SouzaGiovanni M Pauletti
Jun 4, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yuzhao Zhou, Robert A Yokel
Apr 2, 2004·Chemical & Pharmaceutical Bulletin·Shabana Iqrar KhanLarry Anthony Walker
Aug 1, 2012·Biopharmaceutics & Drug Disposition·José Eduardo GonçalvesSílvia Storpirtis
May 5, 2017·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Yan LiuXiaohong Liu
Jan 22, 2003·In Vitro Cellular & Developmental Biology. Animal·Dennis A LaskaTerry Lindstrom
May 26, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Arianna FediSilvia Scaglione
Apr 9, 2005·International Journal of Pharmaceutics·Nazila Salamat-Miller, Thomas P Johnston
May 3, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Eileen F MurphyKevin D Cashman
Apr 21, 2007·Journal of Agricultural and Food Chemistry·Megumi MatsumotoHiroshi Hara
Mar 17, 2010·Biochemical and Biophysical Research Communications·Zhengrong LiYuhong Xu

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