Characterization of each aefR and mexT mutant in Pseudomonas syringae pv. tabaci 6605.

Molecular Genetics and Genomics : MGG
Yuichiro KawakitaYuki Ichinose

Abstract

To investigate the mechanism of activation of the genes for resistance-nodulation-division (RND) family members MexE, MexF, and OprN for multidrug resistance (MDR), we mutagenized aefR and mexT, the potential regulators of mexEF/oprN transcription in Pseudomonas syringae pv. tabaci 6605 (Pta 6605). AefR is a member of the TetR transcription factors, and is known to be required for production of the quorum-sensing molecules, acyl homoserine lactones (AHL), in P. syringae. Furthermore, we found that AHL-synthesis-defective mutant strains in Pta 6605 showed enhanced expression of mexEF/oprN, and were highly tolerant to antimicrobial compounds such as chloramphenicol. MexT is a LysR-type transcription factor and is known to positively regulate transcription of mexEF/oprN in Pseudomonas aeruginosa. The ∆aefR mutant reduced the amount of growth in in vitro culture, caused the loss of AHL production, reduced the swarming motility, virulence and expression of psyI (AHL synthase) and psyR (AHL transcriptional regulator), and enhanced mexEF/oprN expression and tolerance to chloramphenicol, whereas the ∆mexT mutant retained the ability to produce AHL and did not show remarkable changes in in vitro growth, tolerance to antimicrobial compou...Continue Reading

References

Nov 1, 1995·Canadian Journal of Microbiology·M F AlexeyevT P Croughan
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·S Heeb, D Haas
Jan 13, 2004·Molecular Plant-microbe Interactions : MPMI·Antje BurseMatthias S Ullrich
May 15, 2004·Molecular Plant-microbe Interactions : MPMI·Beatriz QuiñonesSteven E Lindow
Jun 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Juan L RamosRaquel Tobes
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·Beatriz QuiñonesSteven E Lindow
Apr 9, 2008·Applied and Environmental Microbiology·Savina O StoitsovaHelge Weingart
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Xin DengXiaoyan Tang
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Nov 16, 2010·Journal of Bacteriology·Yongxin JinShouguang Jin
Dec 18, 2010·Molecular Genetics and Genomics : MGG·Eiko KandaYuki Ichinose
Jun 9, 2011·Molecular Plant-microbe Interactions : MPMI·Paola VargasMaría-Trinidad Gallegos

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