Abstract
Human neonates are immunologically immature, particularly in their humoral antibody responses to T cell-independent antigens, as exemplified by their increased susceptibility to infections with polysaccharide-encapsulated bacteria. To clarify the mechanism(s) underlying the unresponsiveness of neonates to polysaccharide antigens, we used an in vitro model with neonatal cord blood cells that has been shown to mimic surface Ig-dependent signaling in the adult by T cell-independent antigens. We studied the ability of cord blood human B cells to become activated after ligation of their surface Ig by unconjugated anti-Ig, dextran-conjugated anti-Ig, and Staphylococcus aureus Cowan A1, and compared their response with that of adult B cells. After the addition of nanogram concentrations of anti-Ig-dextran, neonatal cord blood B cells proliferated at levels comparable to that observed with adult B cells. The majority of cord blood B cells showed a marked rise in intracellular calcium, increased surface expression of human leukocyte antigen DR, and an increase in cell size. Direct activation of protein kinase C by phorbol esters in neonatal B cells led to cellular proliferation, and when combined with anti-Ig, a synergistic effect on pr...Continue Reading
References
Mar 1, 1976·Clinical Immunology and Immunopathology·L Y WuA R Lawton
Feb 1, 1992·International Immunology·N M BhatN N Teng
Aug 1, 1990·European Journal of Immunology·G T ReheJ J Mond
Oct 1, 1990·Clinical and Experimental Immunology·G PastorelliJ E de Vries
Mar 1, 1990·The Journal of Clinical Investigation·D S PisetskyP E Lipsky
Jan 1, 1990·Scandinavian Journal of Immunology·P M LydyardD Isenberg
Oct 1, 1986·Immunological Reviews·L A HerzenbergL A Herzenberg
Oct 1, 1987·Molecular Immunology·C J Lee
Nov 1, 1985·Scandinavian Journal of Immunology·H C CleversR E Ballieux
Aug 31, 1967·The New England Journal of Medicine·C A AlfordG Cassady
Apr 1, 1968·The American Journal of Medicine·M D CooperR A Good
May 1, 1984·Cellular Immunology·L M WahlS M Wahl
May 1, 1984·Cellular Immunology·S M WahlL M Wahl
Aug 1, 1980·The Journal of Clinical Investigation·G TosatoR M Blaese
Nov 1, 1980·European Journal of Immunology·U AnderssonS Britton
Jan 1, 1983·European Journal of Immunology·U AnderssonG Bird
May 1, 1983·European Journal of Immunology·M J Van TolR E Ballieux
May 1, 1983·Acta paediatrica Scandinavica·S A PedersenV Andersen
Jan 1, 1981·Immunological Reviews·U AnderssonR Palacios
Jul 1, 1983·The Journal of Infectious Diseases·J C Hill
Oct 1, 1980·The Journal of Pediatrics·H F Pabst, H W Kreth
Dec 1, 1980·The Journal of Infectious Diseases·B M GrayH C Dillon
Sep 1, 1995·Pediatric Research·H ZolaD Roberton
Nov 1, 1995·The Journal of Infectious Diseases·H KäyhtyJ Eskola
Mar 1, 1995·American Journal of Public Health·A M LoughlinS M Lett
Jan 1, 1995·Biology of the Neonate·A A El-MohandesI M Katona
Apr 1, 1997·International Immunology·L Tasker, S Marshall-Clarke
Citations
Nov 24, 2001·Resuscitation·T HoehnC Bührer
Feb 13, 2001·Immunology·J KovarikC A Siegrist
Feb 22, 2003·Infection and Immunity·Guoling XiDiane Wallace Taylor
Jan 26, 2010·Human Immunology·Nima RezaeiRobert C Read
Mar 23, 2005·Vaccine·Kerrie VaughanLaurel J Gershwin
Nov 21, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martha A Alexander-Miller
Nov 25, 2003·Clinical and Experimental Immunology·L Tasker, S Marshall-Clarke