PMID: 9525641Apr 3, 1998Paper

Characterization of Ebola virus entry by using pseudotyped viruses: identification of receptor-deficient cell lines

Journal of Virology
R J Wool-Lewis, P Bates

Abstract

Studies analyzing Ebola virus replication have been severely hampered by the extreme pathogenicity of this virus. To permit analysis of the host range and function of the Ebola virus glycoprotein (Ebo-GP), we have developed a system for pseudotyping these glycoproteins into murine leukemia virus (MLV). This pseudotyped virus, MLV(Ebola), can be readily concentrated to titers which exceed 5 x 10(6) infectious units/ml and is effectively neutralized by antibodies specific for Ebo-GP. Analysis of MLV(Ebola) infection revealed that the host range conferred by Ebo-GP is very broad, extending to cells of a variety of species. Notably, all lymphoid cell lines tested were completely resistant to infection; we speculate that this is due to the absence of a cellular receptor for Ebo-GP on B and T cells. The generation of high-titer MLV(Ebola) pseudotypes will be useful for the analysis of immune responses to Ebola virus infection, development of neutralizing antibodies, analysis of glycoprotein function, and isolation of the cellular receptor(s) for the Ebola virus.

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Citations

Nov 26, 2003·Antimicrobial Agents and Chemotherapy·Fátima LasalaRafael Delgado
Feb 24, 2004·Infection and Immunity·Wolfram R ZückertAlan G Barbour
Mar 30, 2005·Journal of Virology·Balaji ManicassamyLijun Rong
Jul 30, 2005·Journal of Virology·Victoria M Wahl-JensenHans-Joachim Schnittler
Feb 28, 2006·Journal of Virology·Séverine BärMarc Alizon
Dec 6, 2007·The Journal of Infectious Diseases·Ayato TakadaYoshihiro Kawaoka
Jun 24, 2008·Critical Reviews in Biochemistry and Molecular Biology·Judith M WhiteKathryn Schornberg
Sep 8, 2010·Proceedings of the National Academy of Sciences of the United States of America·Derek DubeJudith M White
Sep 24, 2010·Clinical and Vaccine Immunology : CVI·Eri NakayamaAyato Takada
Oct 19, 2011·The Journal of Infectious Diseases·Osvaldo MartinezChristopher F Basler
May 23, 2012·Expert Opinion on Drug Discovery·Sheli R RadoshitzkySina Bavari
Mar 8, 2012·The EMBO Journal·Emily Happy MillerKartik Chandran
Dec 4, 2012·Expert Review of Anti-infective Therapy·Yanchen Zhou, Graham Simmons
Jul 4, 2013·Journal of Biomolecular Screening·Juan WangLijun Rong
Oct 10, 2014·The Journal of Pathology·Roosecelis Brasil MartinesSherif R Zaki
Apr 17, 2015·The Journal of Infectious Diseases·Franziska DahlmannHeike Hofmann-Winkler
Oct 30, 2015·Viruses·Sandra L Bixler, Arthur J Goff
Mar 1, 2016·Uirusu·Yasuteru Sakurai
Sep 2, 2016·Virology Journal·Lisa HenßBarbara S Schnierle
Oct 30, 2016·Journal of Virology·Hadas Cohen-DvashiRon Diskin
Apr 20, 2017·Nature Reviews. Microbiology·Andreas S PuschnikJan E Carette
Jan 22, 2020·PLoS Neglected Tropical Diseases·Marcel BokelmannAndreas Kurth
Jul 29, 2020·Proceedings of the National Academy of Sciences of the United States of America·Nicolás Sarute, Susan R Ross
May 3, 2001·Journal of Virology·L D JasenoskyY Kawaoka
Apr 23, 2002·Journal of Virology·Cyril J Empig, Mark A Goldsmith
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·Graham SimmonsPaul Bates

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